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| Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1994Articles/phyto84n05_541.pdf#page=9
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Wetzel, H. C.;
Dernoeden, P. H.;
Millner, P. D. |
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| Author Affiliation: | Department of Agronomy, The University of Maryland, College Park, MD |
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| Title: | Identification of ectotrophic root-infecting (ERI) fungi of turfgrasses with the random amplified polymorphic DNA (RAPD) assay |
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| Meeting Info.: | Joint Meeting of the APS Potomac and Northeastern Divisions: Held: February 9-11, 1994, Carlisle, PA |
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| Source: | Phytopathology. Vol. 84, No. 5, May 1994, p. 549. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Turfgrasses; Disease identification; Patch diseases; Fungi; DNA
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| Abstract/Contents: | "Turfgrass patch diseases caused by ERI fungi are among the most devastating in the U.S. The precise identification of ERI fungi can be difficult and time consuming because it requires laboratory isolation and culture to produce ascocarps. Production of the sexual stage in the laboratory is frequently unsuccessful because incompatible mating types may have been isolated or specific inducers of the sexual stage are unknown. Since these fungi can be isolated from their hosts, tenative identifications are currently made on the basis of cultural morphological characteristics. To reduce the dependence on phenotypic characteristics of ERI fungi, establishment of a new technique of identification that relies on inherent genotypic characteristics of these organisms is desirable. We investigated the use of RAPD for the identification of the ERI fungi. Initial RAPD reactions consisted of screening 37 reference isolates of five genera against 16 primers (10-base oligonucleotides). Characterization of the RAPD fingerprints of 20 unknown ERI fungi isolated from symptomatic turfgrass plants. Comparison of RAPD fingerprints between the reference and unknown isolates showed that ERI fungi could be distinguished at the species and variety levels." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wetzel, H. C., P. H. Dernoeden, and P. D. Millner. 1994. Identification of ectotrophic root-infecting (ERI) fungi of turfgrasses with the random amplified polymorphic DNA (RAPD) assay. Phytopathology. 84(5):p. 549. |
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| Web URL(s):
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1994Articles/phyto84n05_541.pdf#page=9
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 599 .P48 |
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