Full TGIF Record # 30638
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Web URL(s):http://www.nrcresearchpress.com/doi/pdf/10.1139/b94-045
    Last checked: 09/29/2015
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Publication Type:
i
Refereed
Author(s):O'Gorman, D.; Xue, B.; Hsiang, T.; Goodwin, P. H.
Author Affiliation:Department of Environmental Biology, University of Guelph, ON, Canada
Title:Detection of Leptosphaeria korrae with the polymerase chain reaction and primers from the ribosomal internal transcribed spacers
Source:Canadian Journal of Botany. Vol. 72, No. 3, March 1994, p. 342-346.
Publishing Information:Vancouver, British Columbia: The National Research Council of Canada.
# of Pages:5
Related Web URL:http://www.nrcresearchpress.com/doi/abs/10.1139/b94-045
    Last checked: 09/29/2015
    Notes: English abstract only
Keywords:TIC Keywords: Ophiosphaerella korrae; Necrotic ring spot; Poa pratensis; DNA
Abstract/Contents:"Leptosphaeria korrae, the causal agent of necrotic ring spot, is a destructive patch disease of Kentucky bluegrass. To develop a rapid molecular test for the detection of this pathogen, an assay based on the polymerase chain reaction (PCR) was developed utilizing the interna transcribed spacer region 1 (ITS 1) of L. korrae ribosomal DNA. DNA sequence comparison showed 94.8% similarity of the ITS 1 region among L. korrae isolates and only 45-50% similarity between L. korrae and other fungal species. Based on ITS 1 sequence differences, a pair of oligonucleotide primers, LK17S and 5.8SC, were selected. With PCR, the primers specifically amplified L. korrae DNA and did not amplify DNA isolated from 15 other fungal species or healthy Kentucky bluegrass. The assay could also specifically detect L. korrae in diseased turfgrass samples."
Language:English
References:33
Note:Abstract also appears in French
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
O'Gorman, D., B. Xue, T. Hsiang, and P. H. Goodwin. 1994. Detection of Leptosphaeria korrae with the polymerase chain reaction and primers from the ribosomal internal transcribed spacers. Can. J. Bot. 72(3):p. 342-346.
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http://www.nrcresearchpress.com/doi/pdf/10.1139/b94-045
    Last checked: 09/29/2015
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
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