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Web URL(s): | https://journals.ashs.org/hortsci/view/journals/hortsci/29/6/article-p624.xml?rskey=8IGfr7 Last checked: 11/12/2019 Requires: PDF Reader |
Publication Type:
| Refereed |
Author(s): | Sweeney, Patricia M.;
Danneberger, T. Karl |
Author Affiliation: | Department of Agronomy, Ohio State University, Columbus, OH |
Title: | Random amplified polymorphic DNA in perennial ryegrass: A comparison of bulk samples vs. individuals |
Source: | HortScience. Vol. 29, No. 6, June 1994, p. 624-626. |
Publishing Information: | St. Joseph, MI: American Society for Horticultural Science |
# of Pages: | 3 |
Keywords: | TIC Keywords: Lolium perenne; Cultivar evaluation; Evaluative methods; SDS-PAGE; DNA
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Abstract/Contents: | "The usefulness of random amplified polymorphic DNA (RAPD) in characterizing two perennial ryegrass (Lolium perenne L.) synthetic cultivars, 'Accolade' and 'Caravelle', was tested. Two out of 10 arbitrary primers produced three RAPD markers that distinguished bulk samples of 30 seedlings. Additional fragments were apparent when DNA from individual seedlings was amplified. Amplification products from bulk samples were not simply the sum of amplification products of individual seedlings and did not reflect all the diversity within or between the cultivars. The study illustrates the need to screen individuals to accurately evaluate the genotypic composition of a synthetic cultivar or heterogeneous population." |
Language: | English |
References: | 14 |
Note: | Figures Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Sweeney, P. M., and T. K. Danneberger. 1994. Random amplified polymorphic DNA in perennial ryegrass: A comparison of bulk samples vs. individuals. HortScience. 29(6):p. 624-626. |
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| Web URL(s): https://journals.ashs.org/hortsci/view/journals/hortsci/29/6/article-p624.xml?rskey=8IGfr7 Last checked: 11/12/2019 Requires: PDF Reader |
| MSU catalog number: SB 1 .H64 |
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