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| DOI: | 10.1111/grs.12217 |
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| Web URL(s): | https://onlinelibrary.wiley.com/doi/full/10.1111/grs.12217
Last checked: 07/17/2019
https://onlinelibrary.wiley.com/doi/pdf/10.1111/grs.12217
Last checked: 07/17/2019
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Author(s): | González-Muñoz, Sergio S.;
Zavaleta-Mancera, Hilda A.;
Miranda-Romero, Luis A.;
Loera-Corral, Octavio;
Pinos-RodrĂguez, Juan M.;
Campos-Montiel, Rafael G.;
Almarez-BuendĂa, Issac |
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| Author Affiliation: | González-Muñoz and Zavaleta-Mancera: College of Postgraduates, Texcoco, Mexico State, Mexico; Miranda-Romero: Zootecnia, Universidad AutĂłnoma Chapingo, Texcoco, Estado de MĂ©xico, MĂ©xico; Loera-Corral: BiotecnologĂa, Universidad AutĂłnoma Metropolitana-Iztapalapa, Iztapalapa, Ciudad de MĂ©xico, MĂ©xico; Pinos-RodrĂguez: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Veracruz, Veracruz, MĂ©xico; Campos-Montiel: Instituto de Ciencias Agropecuarias, Universidad AutĂłnoma del Estado de Hidalgo, Tulancingo, Hidalgo, MĂ©xico; Almarez- BuendĂa: College of Postgraduates, Texcoco, Mexico State, Mexico and Instituto de Ciencias Agropecuarias, Universidad AutĂłnoma del Estado de Hidalgo, Tulancingo, Hidalgo, MĂ©xico |
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| Title: | Histochemical changes in early and mature Festulolium and maturation's effects on rumen bacteria activity and in vitro degradation |
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| Source: | Grassland Science. Vol. 65, No. 1, January 2019, p. 23-31. |
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| Publishing Information: | Oxford, England: Blackwell Pub. |
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| # of Pages: | 9 |
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| Keywords: | TIC Keywords: Cell wall; Degradation; Electron microscopy; Fermentation; Festulolium; Histochemical methods; In vitro; Lignification; Maturation; Quality evaluation; Staining; Tissue testing
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| Abstract/Contents: | "The nutritional quality of grasses decreases with maturity due to increases in content of cell wall (CW) and lignified tissue, which affect the degradation and fermentation variables of the grasses. The effect of leaf age and tissue-microbial attachment during the degradation of Festulolium at 28 and 35 days of regrowth was evaluated in vitro by gas production. Fragments from the middle part of the blade of Festulolium leaves were stained for lignin with phloroglucinol solution, and the lignified area was measured. Subepidermal fibers of the abaxial and adaxial central vein of leaves contributed most to the lignification of Festulolium at 35 days compared with that at 28 days. Variables of gas production and concentrations of volatile fatty acids and ammonia nitrogen were affected by regrowth age. Activity for cellulases (8.19 IU/g dry matter [DM]) was highest between 12 and 16 hr of incubation and for xylanases between 16 and 24 hr (51.09 IU/g DM) without differences between regrowth ages. Scanning electron microscopy showed the following sequence: primary CW degradation started at 12 hr, a large population of microbiota were attached to CWs with extracellular protein complexes at 24 hr, and microbiota were attached to lignified cells at 48 hr. Therefore, during in vitro CW degradation of Festulolium, enzymatic activities increased before microbiota attachment to CWs, which was maintained when xylanase activity declined. Thus, structural analysis conducted using histochemistry and scanning electron microscopy helped to characterize the degradation of plant tissues and the interactions with ruminal microorganisms." |
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| Language: | English |
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| References: | 37 |
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| Note: | Pictures, color & b/w
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
González-Muñoz, S. S., H. A. Zavaleta-Mancera, L. A. Miranda-Romero, O. Loera-Corral, J. M. Pinos-RodrĂguez, R. G. Campos-Montiel, et al. 2019. Histochemical changes in early and mature Festulolium and maturation's effects on rumen bacteria activity and in vitro degradation. Grassland Science. 65(1):p. 23-31. |
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| DOI: 10.1111/grs.12217 |
| Web URL(s):
https://onlinelibrary.wiley.com/doi/full/10.1111/grs.12217
Last checked: 07/17/2019
https://onlinelibrary.wiley.com/doi/pdf/10.1111/grs.12217
Last checked: 07/17/2019
Requires: PDF Reader |
| 
MSU catalog number: b4979016 |
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