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| DOI: | 10.1111/grs.12218 |
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| Web URL(s): | https://onlinelibrary.wiley.com/doi/10.1111/grs.12218
Last checked: 07/17/2019
https://onlinelibrary.wiley.com/doi/pdf/10.1111/grs.12218
Last checked: 07/17/2019
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Author(s): | Kubota, Akito;
Akiyama, Yukio;
Fujimori, Masahiro;
Tamura, Ken-Ichi;
Sasaki, Tohru;
Kasai, Eri |
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| Author Affiliation: | Kubota and Fujimori: National Agriculture and Food Research Organization (NARO), Tohoku Agricultural Research Center, Morioka, Japan; Akiyama and Tamura: National Agriculture and Food Research Organization (NARO), Hokkaido Agricultural Research Center, Sapporo, Japan; Sasaki and Kasai: Forage Crop Research Institute, Japan Grassland Agriculture and Forage Seed Association, Nasushiobara, Japan |
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| Title: | Simple DNA marker-assisted selection of endophyte (Epichloë uncinata)-infected festulolium |
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| Source: | Grassland Science. Vol. 65, No. 1, January 2019, p. 32-40. |
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| Publishing Information: | Oxford, England: Blackwell Pub. |
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| # of Pages: | 8 |
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| Keywords: | TIC Keywords: Backcrossing; Festulolium; Genetic analysis; Genetic markers; Lolium multiflorum; Neotyphodium uncinatum; Polymerase chain reaction; Progeny testing
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| Abstract/Contents: | "In this study, to breed endophyte (Epichloe uncinata)-infected festololium (x Festulolium Aschers.et Graebn.), we backcrossed endophyte-infected Italian ryegrass (Lolium multiflorum Lam.) to festulolium. To prevent self-pollinated plants being mixed with the progeny, we used polymerase chain reaction (PCR)-based DNA marker sets distinguishing between Lolium and Festuca species. In the first backcrossing (F1 development), 20 of the 21 progeny plants showed Festuca-specific band patterns and were endophyte-infected. We developed six maternal lines (BC1 development) by backcrossing six selected F1 plants. More than 97% of plants from five maternal lines had novel Festuca-specific band patterns which their maternal parents lacked. However, only 54% plants of the maternal line F1-18 exhibited novel Festuca-specific band patterns. F1-18 showed Festuca-specific band patterns with almost all tested DNA marker sets, and its f ratio (the ratio of Festuca-specific region to the whole genome) was 31.1%, indicating that F1-18 had an almost complete set of the Festuca genome. Because paternal backcrossing festulolium parents were amphidiploids, an f ratio of approximately 25% corresponds to a complete set of the Festuca genome. We found that it was difficult to evaluate BC2 hybridity using the same DNA marker sets because very few DNA markers were distinctive. In total, we bred and selected at least 146 true BC1 hybrids and endophyte-infected plants using DNA markers." |
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| Language: | English |
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| References: | 22 |
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| Note: | Pictures, color & b/w
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Kubota, A., Y. Akiyama, M. Fujimori, K.-i. Tamura, T. Sasaki, and E. Kasai. 2019. Simple DNA marker-assisted selection of endophyte (Epichloë uncinata)-infected festulolium. Grassland Science. 65(1):p. 32-40. |
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| DOI: 10.1111/grs.12218 |
| Web URL(s):
https://onlinelibrary.wiley.com/doi/10.1111/grs.12218
Last checked: 07/17/2019
https://onlinelibrary.wiley.com/doi/pdf/10.1111/grs.12218
Last checked: 07/17/2019
Requires: PDF Reader |
| 
MSU catalog number: b4979016 |
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