Full TGIF Record # 309454
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Web URL(s):https://scisoc.confex.com/scisoc/2019am/meetingapp.cgi/Paper/119685
    Last checked: 11/26/2019
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Publication Type:
Content Type:Abstract or Summary only
Author(s):Rushford, Clayton A.; Miller, Gerald L.
Author Affiliation:Rushford: University of Missouri-Columbia, Columbia, MO; Miller: Plant Sciences, University of Missouri, Columbia, MO
Title:Pythium dissemination through golf course irrigation systems
Section:C05 turfgrass science
Other records with the "C05 turfgrass science" Section

Turfgrass pest management oral 1 (includes student competition)
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Meeting Info.:San Antonio, Texas: November 10-13, 2019
Source:ASA, CSSA and SSSA International Annual Meetings. 2019, p. 119685.
# of Pages:1
Publishing Information:[Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America]
Keywords:TIC Keywords: Agrostis stolonifera; Culture collections; DNA extraction; Disease identification; Golf course irrigation; Pythium; Water resources; Water testing
Abstract/Contents:"Creeping bentgrass is often used on golf putting greens, and is especially prone to diseases caused by Pythium spp. These pathogens spread through irrigation in agricultural and greenhouse settings, but dissemination through golf course irrigation is unknown. Water samples (1.5 L) were collected from irrigation heads on ten golf courses in April and July of 2018 and 2019. A subset of these locations was sampled in October of 2018. Except for April 2018, water sources (retention ponds, wells, a lake, and municipal water) were also sampled. Culture-based detection methods included baiting with creeping bentgrass leaves and membrane filtration. Cultureless detection methods involved DNA extraction from bait tissue and filters. For identification, DNA extracts were amplified using oomycete-specific primers, with amplicons subsequently sequenced. Amplicons were phylogenetically compared with Genbank accessions. Six Pythium spp. have been putatively identified based on sequence comparison. Nonpathogenic species included P. plurisporium and P. torulosum. Other species with unknown pathogenicity to creeping bentgrass include P. flevoense, P. adhaerens, P. monospermum, and P. rostratifingins. The latter three are known causal agents of root rot in other plant hosts. Pythium adhaerens, P. monospermum, and P. torulosum were detected every month, while P. flevoense, P. plurisporium, and P. rostratifingins were only detected in April. All six species were detected in irrigation heads supplied by retention ponds, while P. adhaerens, P. flevoense, and P. torulosum were also detected in samples taken directly from retention ponds. Pythium adhaerens and P. torulosum were detected in irrigation heads supplied by wells. Pythium adhaerens and P. monospermum were detected in irrigation heads supplied by lake water. No Pythium spp. were detected in municipal water systems. Cultureless methods were often unsuccessful because many samples contained multiple species. These results suggest persistent dissemination of Pythium spp. in irrigation from non-municipal water sources."
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Rushford, C. A., and G. L. Miller. 2019. Pythium dissemination through golf course irrigation systems. Agron. Abr. p. 119685.
Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=309454
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