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Web URL(s): | https://scisoc.confex.com/scisoc/2019am/meetingapp.cgi/Paper/118760 Last checked: 12/05/2019 Requires: JavaScript |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Brown, Jessica;
Yu, Xingwang;
Tuong, Tan D.;
Livingston, David;
Milla-Lewis, Susana R. |
Author Affiliation: | Brown: North Carolina State University, Raleigh, NC; Yu and Milla-Lewis: Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC; Tuong and Livingston: U.S. Department of Agriculture, Raleigh, NC |
Title: | Transcriptomic approach to identification of freezing tolerance components in zoysiagrass |
Section: | C05 turfgrass science Other records with the "C05 turfgrass science" Section
Molecular techniques, genetics and plant breeding oral (includes student competition) Other records with the "Molecular techniques, genetics and plant breeding oral (includes student competition)" Section
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Meeting Info.: | San Antonio, Texas: November 10-13, 2019 |
Source: | ASA, CSSA and SSSA International Annual Meetings. 2019, p. 118760. |
Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America] |
# of Pages: | 1 |
Keywords: | TIC Keywords: Cold acclimation; Cold resistance; Gene expression; Genetic analysis; Ribonucleic acid; Transcription factors; Zoysia japonica
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Cultivar Names: | Victoria; Meyer |
Abstract/Contents: | "Zoysiagrass (Zoysia spp.) are warm season turfgrasses desirable for recreational and ornamental purposes due to their relatively low input requirements and wide general tolerance to drought, shade and salinity. Although the most freeze tolerant of the warm season grasses, the commercial spread of zoysiagrass is mostly limited to the transition zone and the southern US. The leading freeze tolerant cultivar, Meyer, was developed in the 1950's, and although some progress has been made in breeding for freeze tolerance in zoysiagrass, the pool of cultivars with high levels of freeze tolerance is still limited. To deepen our understanding of the physiologic and metabolic components of freezing tolerance in zoysiagrass, a transcriptomic approach was utilized to identify genes involved in cold acclimation, a critical step in the development of freezing tolerance. Meyer, a freeze tolerant cultivar, and Victoria, a free susceptible cultivar were used to assess transcriptomic response to cold acclimation. Ninety-six pots, forty-eight pots per cultivar, were subjected to either a cold acclimation or non-cold acclimation treatment. Leaf samples collected after 48 hours into treatment were taken and the RNA was extracted, then sequenced. Illumina sequencing results were analyzed in correspondence with the zoysiagrass reference genome to determine differential gene expression. Genes of interest for cold acclimation were identified." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! "218-5" |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Brown, J., X. Yu, T. D. Tuong, D. Livingston, and S. R. Milla-Lewis. 2019. Transcriptomic approach to identification of freezing tolerance components in zoysiagrass. Agron. Abr. p. 118760. |
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