Full TGIF Record # 309594
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Web URL(s):https://scisoc.confex.com/scisoc/2019am/meetingapp.cgi/Paper/122001
    Last checked: 12/05/2019
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Publication Type:
Content Type:Abstract or Summary only
Author(s):Catching, Katherine E.; Raymer, Paul L.
Author Affiliation:Catching: Institute of Plant Breeding, Genetics and Genomics, Humboldt State University, Santa Rosa, CA; Raymer: University of Georgia-Griffin, Griffin, GA
Title:Engineering dollar spot resistance in Paspalum vaginatum (seashore paspalum) by biolistic gene transformation
Section:C05 turfgrass science
Other records with the "C05 turfgrass science" Section

Molecular techniques, genetics and plant breeding oral (includes student competition)
Other records with the "Molecular techniques, genetics and plant breeding oral (includes student competition)" Section
Meeting Info.:San Antonio, Texas: November 10-13, 2019
Source:ASA, CSSA and SSSA International Annual Meetings. 2019, p. 122001.
Publishing Information:[Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America]
# of Pages:1
Keywords:TIC Keywords: Biolistic transformation; Cultivar improvement; Disease resistance; Dollar spot; Genetic engineering; Oxalate oxidase; Paspalum vaginatum; Promoters
Abstract/Contents:"Disease resistance to fungal pathogens is a highly desirable trait in both crop and ornamental plants. In the case of Paspalum vaginatum (seashore paspalum) a warm-season turf grass species that is ideal for golf and sports fields, dollar spot is the most destructive and costly disease to manage. Dollar spot is caused by Clarireedia homoeocarpa, a non-sporulating fungal pathogen that damages turfgrasses by exuding oxalic acid and colonizing the damaged tissue. Our objective is to develop seashore paspalum with dollar-spot resistance by using a gene for oxalate-oxidase to neutralize the oxalic acid. Towards that end, a wheat germin gene, gf 2.8, will be incorporated via biolistic transformation. Two promoters, CaMV35S and ZmUb, will be evaluated for their ability to drive gf 2.8 with the rice catalase terminator. Negative and positive selection efficiency will be compared using hygromycin B and arabitol. Six week old embryogenic callus obtained from nodal explants will be used for biolistic transformation. Disease resistance will then be quantified through field trials and oxidase assays."
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Catching, K. E., and P. L. Raymer. 2019. Engineering dollar spot resistance in Paspalum vaginatum (seashore paspalum) by biolistic gene transformation. Agron. Abr. p. 122001.
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