Full TGIF Record # 309597
Item 1 of 1
Web URL(s):https://scisoc.confex.com/scisoc/2019am/meetingapp.cgi/Paper/119574
    Last checked: 12/05/2019
    Requires: JavaScript
Publication Type:
Content Type:Abstract or Summary only
Author(s):Qiu, Yinjie; Hirsch, Cory; Watkins, Eric
Author Affiliation:Qiu and Watkins: Department of Horticultural Science, University of Minnesota-Twin Cities, St. Paul, MN; Hirsch: Department of Plant Pathology, University of Minnesota, Saint Paul, MN
Title:Unveiling transcriptome composition in hexaploid hard fescue (Festuca brevipila) through PacBio isoform sequencing
Section:C05 turfgrass science
Other records with the "C05 turfgrass science" Section

Molecular techniques, genetics and plant breeding oral (includes student competition)
Other records with the "Molecular techniques, genetics and plant breeding oral (includes student competition)" Section
Meeting Info.:San Antonio, Texas: November 10-13, 2019
Source:ASA, CSSA and SSSA International Annual Meetings. 2019, p. 119574.
Publishing Information:[Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America]
# of Pages:1
Keywords:TIC Keywords: Festuca brevipila; Fungicide evaluation; Gene expression; Genetic analysis; Hexaploid; Propiconazole
Abstract/Contents:"Hard fescue (Festuca brevipila Tracey, 2n=6x=42) is a fine-textured, cool-season turfgrass that does well under low-input management. Breeding and genetics studies of F. brevipila have been limited due to the complex nature of its hexaploid genome. To advance our knowledge of F. brevipila, we used PacBio isoform sequencing to develop a reference transcriptome of this taxon. Here, we report the F. brevipila reference transcriptome generated from root, crown, leaf, and seed head tissues using 4 SMRT cells. We obtained 59,510 full-length transcripts and further refined them into 38,595 non-redundant full-length transcripts which had a N50 of 2,585 bp. The longest and shortest transcripts were 11,487 and 58 bp, respectively. Transcriptome annotation using NCBI NR protein and Uniprot databases returned 36,075 (93.47%) and 29,670 (76.88%) annotated transcripts, respectively. Finally, we reconstructed the cytochrome P450 gene family phylogenetic tree of F. brevipila to study the effect of propiconazole fungicide on this complex gene family. We found gene expression of CYP709B3 and CYP94C1 had been affected 6 days post the propiconazole treatment. We also identified several gene expression changes in the fatty acid elongation pathway and genes related to histone modifications. Overall, this F. brevipila reference transcriptome will provide geneticists with a foundation to further study this important turfgrass taxon."
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Qiu, Y., C. Hirsch, and E. Watkins. 2019. Unveiling transcriptome composition in hexaploid hard fescue (Festuca brevipila) through PacBio isoform sequencing. Agron. Abr. p. 119574.
Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=309597
If there are problems with this record, send us feedback about record 309597.
Choices for finding the above item:
Web URL(s):
    Last checked: 12/05/2019
    Requires: JavaScript
Find from within TIC:
   Digitally in TIC by record number.
Request through your local library's inter-library loan service (bring or send a copy of this TGIF record)