Full TGIF Record # 31064
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Web URL(s):https://www.crops.org/publications/cs/pdfs/36/5/CS0360051301
    Last checked: 08/05/2010
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Publication Type:
i
Refereed
Author(s):Sweeney, P. M.; Danneberger, T. K.
Author Affiliation:Department of Horticulture and Crop Science, The Ohio State University, Columbus, Ohio
Title:Restriction digestion of arbitrary amplification fragments of annual bluegrass
Section:Turfgrass Science
Other records with the "Turfgrass Science" Section
Source:Crop Science. Vol. 36, No. 5, September/October 1996, p. 1301-1303.
Publishing Information:Madison, WI: Crop Science Society of America
# of Pages:3
Keywords:TIC Keywords: Poa annua turf; Genetics; Breeding improvement; Enzymes; Genetic markers
Abstract/Contents:"The production of co-dominant markers by digesting arbitrarily primed amplification fragments with restriction endonucleases would be useful in genetics and plant breeding studies. The feasibility of generating restriction amplification fragment length polymorphism (RAFLP) for use as genetic markers for annual bluegrass (Poa annua L.) was evaluated. Five restriction enzymes that (HindIII, Kpnl, PstI,ApaI, and SmaI) successfully digested DNA in the amplification buffer tested were used to digest arbitrarily amplified DNA from P. annua. These restriction enzymes used in conjuction with 53 primers identified 82 restriction loci. Evaluation of 44 loci revealed three RAFLP markers. These and other RAFLP markers should be useful as co-dominant genetic markers for P. annua and other species where few genetic markers exist, little genomic information is available, or tissue is limited.
Language:English
References:13
Note:Pictures, b/w
Figures
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Sweeney, P. M., and T. K. Danneberger. 1996. Restriction digestion of arbitrary amplification fragments of annual bluegrass. Crop Sci. 36(5):p. 1301-1303.
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Web URL(s):
https://www.crops.org/publications/cs/pdfs/36/5/CS0360051301
    Last checked: 08/05/2010
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
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MSU catalog number: SB 183 .C7
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