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Web URL(s): | https://www.crops.org/publications/cs/pdfs/36/5/CS0360051301 Last checked: 08/05/2010 Requires: PDF Reader Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Sweeney, P. M.;
Danneberger, T. K. |
Author Affiliation: | Department of Horticulture and Crop Science, The Ohio State University, Columbus, Ohio |
Title: | Restriction digestion of arbitrary amplification fragments of annual bluegrass |
Section: | Turfgrass Science Other records with the "Turfgrass Science" Section
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Source: | Crop Science. Vol. 36, No. 5, September/October 1996, p. 1301-1303. |
Publishing Information: | Madison, WI: Crop Science Society of America |
# of Pages: | 3 |
Keywords: | TIC Keywords: Poa annua turf; Genetics; Breeding improvement; Enzymes; Genetic markers
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Abstract/Contents: | "The production of co-dominant markers by digesting arbitrarily primed amplification fragments with restriction endonucleases would be useful in genetics and plant breeding studies. The feasibility of generating restriction amplification fragment length polymorphism (RAFLP) for use as genetic markers for annual bluegrass (Poa annua L.) was evaluated. Five restriction enzymes that (HindIII, Kpnl, PstI,ApaI, and SmaI) successfully digested DNA in the amplification buffer tested were used to digest arbitrarily amplified DNA from P. annua. These restriction enzymes used in conjuction with 53 primers identified 82 restriction loci. Evaluation of 44 loci revealed three RAFLP markers. These and other RAFLP markers should be useful as co-dominant genetic markers for P. annua and other species where few genetic markers exist, little genomic information is available, or tissue is limited. |
Language: | English |
References: | 13 |
Note: | Pictures, b/w Figures |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Sweeney, P. M., and T. K. Danneberger. 1996. Restriction digestion of arbitrary amplification fragments of annual bluegrass. Crop Sci. 36(5):p. 1301-1303. |
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| Web URL(s): https://www.crops.org/publications/cs/pdfs/36/5/CS0360051301 Last checked: 08/05/2010 Requires: PDF Reader Access conditions: Item is within a limited-access website |
| MSU catalog number: SB 183 .C7 |
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