Full TGIF Record # 310813
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Web URL(s):https://archive.lib.msu.edu/tic/ressum/2019/2019.pdf#page=16
    Last checked: 04/14/2020
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Publication Type:
i
Report
Author(s):Luo, Hong
Author Affiliation:Department of Genetics and Biochemistry, Clemson University
Title:Genetic engineering of turfgrass for enhanced multi-stress resistance
Section:Genetics and breeding
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Cool-season grasses
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Source:Turfgrass and Environmental Research Program: 2019 Research Summaries. 2019, p. 8-12.
Publishing Information:[New York, New York]: The United States Golf Association Green Section
# of Pages:5
Keywords:TIC Keywords: Breeding aims; Drought resistance; Genetic engineering; Heat resistance; Nutrient deficiency
Language:English
References:0
See Also:Other Reports from this USGA research project: 2016-25-575
Note:Pictures, color
USGA Summary Points:Three representative transgenic lines harboring the chimeric gene construct, p35S-AVP1/Ubi-OsSIZ1/Ubi-FNR:Fld/p35S-bar, and expressing high level of the three stress-related genes, AVP1, OsSIZ1 and Fld were grown in greenhouse and asexually multiplied for performance evaluation. Another three transgenic lines expressing AVP1, OsSIZ1 and Fld, respectively, as well as non-transgenic wild type (WT) plants were also simultaneously grown in greenhouse and used as controls for comparison. Assessed plant growth under normal conditions comparing different transgenic lines and wild type plants and observed that the transgenic lines expressing three genes together (AVP1, OsSIZ1 and Fld) outperformed wild type and transgenic plants expressing only one of the three genes (AVP1, OsSIZ1 and Fld), exhibiting rapid plant growth with more biomass production (examples shown in Fig. 1). Conducted salinity and drought stress and nitrogen deficiency tolerance test with all the transgenic lines and wild type plants. The transgenic lines expressing three genes together (AVP1, OsSIZ1 and Fld) outperformed wild type and transgenic plants expressing only one of the three genes (AVP1, OsSIZ1 and Fld), exhibiting enhanced tolerance to the stress conditions tested (examples shown in Fig. 2 & 3). Our data showed that simultaneous introduction of three transgenes into turfgrass not only boosted plant growth, but also significantly enhanced plant performance under various adverse environmental conditions. Assessment of plant response to additional abiotic stresses including oxidative stress, P starvation and study of other characteristics are currently being completed. The results obtained demonstrate the great potential of extending the same strategy to other turf and forage species as well as vegetable and food crops, significantly enhancing agriculture production. The plant materials could be directly used for cultivation upon completion of the government dregulation process, or used as foundational breeding materials for products of wide environmental adaptation.
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Luo, H. 2019. Genetic engineering of turfgrass for enhanced multi-stress resistance. USGA Turfgrass Environ. Res. Summ. p. 8-12.
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Web URL(s):
https://archive.lib.msu.edu/tic/ressum/2019/2019.pdf#page=16
    Last checked: 04/14/2020
    Requires: PDF Reader
    Notes: Item is within a single large file
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MSU catalog number: b3609415
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