Full TGIF Record # 31092
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Author(s):Freeman, G. W.; Yoder, F. A. Jr.
Author Affiliation:New Jersey Department of Agiculture, Trenton, NJ
Title:Esterase differences in seedlings and seedling mixtures of hard and red fescues
Source:Seed Science and Technology. Vol. 22, No. 1, 1994, p. 27-31.
# of Pages:5
Publishing Information:International Seed Testing Association
Keywords:TIC Keywords: Festuca rubra subsp. rubra; Festuca ovina subsp. duriuscula; Esterase; Seedlings; Seed mixtures
Abstract/Contents:"Rapid and efficient methods are needed to differentiate between hard fescue (Festuca longifolia Thuill.), creeping red fescue (Festuca rubra L. subsp. rubra) and chewings fescue (Festuca rubra L. subsp. commutata). Visual evaluation of seeds and the ammonia root fluorescence test sometimes fail to separate species and subspecies of fescue. This study was initiated to determine if distinct esterase banding patterns provide consistent indicators of the species or subspecies and if the components of mixtures of hard and red fescue seedlings could be detected using polyacrylamide gel electrophoresis (PAGE). Hard and red fescue seedling extracts had distinct esterase isoenzyme banding patterns. The staining intensity of bands at Rf 0.62 to 0.64, indicative of the hard fescues, was used to detect hard fescue in hard fescue in hard fescue-red fescue mixtures. Hard fescue was detected by visual examination of PAGE gels when the proportion of hard fescue seedlings in the mixture was as low as 25%. A band at Rf 0.50 which the creeping red fescue cultivars did not exhibit was useful in differentiating chewings fescues from creeping red fescues."
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Freeman, G. W., and F. A. Jr. Yoder. 1994. Esterase differences in seedlings and seedling mixtures of hard and red fescues. Seed Sci. Technol. 22(1):p. 27-31.
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