Full TGIF Record # 315300
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DOI:10.1111/nph.16528
Web URL(s):https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.16528
    Last checked: 11/02/2020
https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.16528
    Last checked: 11/02/2020
    Requires: PDF Reader
Publication Type:
i
Refereed
Author(s):Green, Kimberly A.; Berry, Daniel; Feussner, Kirstin; Eaton, Carla J.; Ram, Arvina; Mesarich, Carl H.; Solomon, Peter; Feussner, Ivo; Scott, Barry
Author Affiliation:Green, Berry, Eaton, and Scott: School of Fundamental Sciences, Massey University, Palmerston North, New Zealand and Bioprotection Research Centre, Massey University, Palmerston North, New Zealand; Kirstin Feussner: Department of Plant Biochemistry, Albrecht von Haller Institute for Plant Sciences, University of Goettingen, Goettingen, Germany and Service Unit for Metabolomics and Lipidomics, Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, Goettingen, Germany; Ram: School of Fundamental Sciences, Massey University, Palmerston North, New Zealand; Mesarich: Bioprotection Research Centre, Massey University, Palmerston North, New Zealand and School of Agriculture and Environment, Massey University, Palmerston North, New Zealand; Solomon: Research School of Biology, Australian National University, Canberra, Australia; Ivo Feussner: Department of Plant Biochemistry, Albrecht von Haller Institute for Plant Sciences, University of Goettingen, Goettingen, Germany and Service Unit for Metabolomics and Lipidomics, Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, Goettingen, Germany and Department of Plant Biochemistry, Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, Goettingen, Germany
Title:Lolium perenne apoplast metabolomics for identification of novel metabolites produced by the symbiotic fungus Epichloë festucae
Column Name:Full papers
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Section:Research
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Source:New Phytologist. Vol. 227, No. 2, July 2020, p. 559-571.
Publishing Information:Oxford, England, United Kingdom: Blackwell Publishing, for the New Phytologist Trust
# of Pages:13
Related Web URL:https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.16528
    Last checked: 11/2/2020
    Notes: Abstract only
Keywords:Author-Supplied Keywords: Apoplast; Endophyte; Epichloë; Metabolome; Symbiosis
Abstract/Contents:"Epichloë festucae is an endophytic fungus that forms a symbiotic association with Lolium perenne. Here we analysed how the metabolome of the ryegrass apoplast changed uponinfection of this host with sexual and asexual isolates of E. festucae. A metabolite fingerprinting approach was used to analyse the metabolite composition of apoplastic wash fluid from uninfected and infected L. perenne. Metabolites enriched or depleted in one or both of these treatments were identified using a set of interactive tools. A genetic approach in combination with tandem MS was used to identify a novel product of a secondary metabolite gene cluster. Metabolites likely to be present in the apoplast were identified using MARVIS in combination with the BioCyc and KEGG databases, and an in-house Epichloë metabolite database. We were able to identify the known endophyte-specific metabolites, peramine and epichloëcyclins, as well as a large number of unknown markers. To determine whether these methods can be applied to the identification of novel Epichloë-derived metabolites, we deleted a gene encoding a NRPS (lgsA) that is highly expressed in planta. Comparative MS analysis of apoplastic wash fluid from wild-type- vs mutant-infected plants identifed a novel Leu/Ile glycoside metabolite present in the former."
Language:English
References:73
Note:Summary appears as abstract
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Green, K. A., D. Berry, K. Feussner, C. J Eaton, A. Ram, C. H. Mesarich, et al. 2020. Lolium perenne apoplast metabolomics for identification of novel metabolites produced by the symbiotic fungus Epichloë festucae. New Phytol. 227(2):p. 559-571.
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DOI: 10.1111/nph.16528
Web URL(s):
https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.16528
    Last checked: 11/02/2020
https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.16528
    Last checked: 11/02/2020
    Requires: PDF Reader
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