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| DOI: | 10.4039/tce.2020.52 |
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| Web URL(s): | https://www-cambridge-org.proxy1.cl.msu.edu/core/journals//canadian-entomologist/article/development-of-a-single-nucleotide-polymorphismbased-dna-marker-for-fall-armyworm-lepidoptera-noctuidae-biotyping-a-case-study-from-the-fall-armyworm-outbreak-in-sri-lanka/3FA787706686A425402C86943906AF05
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https://www-cambridge-org.proxy1.cl.msu.edu/core/services/aop-cambridge-core/content/view/3FA787706686A425402C86943906AF05/S0008347X20000528a.pdf/development_of_a_single_nucleotide_polymorphismbased_dna_marker_for_fall_armyworm_lepidoptera_noctuidae_biotyping_a_case_study_from_the_fall_armyworm_outbreak_in_sri_lanka.pdf
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| Publication Type:
| Refereed |
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| Author(s): | Nanayakkara, Dhanesha;
Jayatilake, Dimanthi;
Kodithuwakku, Suranga |
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| Author Affiliation: | Nanayakkara and Jayatilake: Department of Agricultural Biology, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka and Kodithuwakku: Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka |
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| Title: | Development of a single nucleotide polymorphism-based DNA marker for fall armyworm (Lepidoptera: Noctuidae) biotyping: A case study from the fall armyworm outbreak in Sri Lanka |
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| Section: | Research papers
Other records with the "Research papers" Section
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| Source: | The Canadian Entomologist. Vol. 152, December 2020, p. 762-771. |
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| Publishing Information: | Toronto, Ontario, Canada: Entomological Society of Canada |
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| # of Pages: | 10 |
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| Related Web URL: | https://www.cambridge.org/core/journals/canadian-entomologist/article/abs/development-of-a-single-nucleotide-polymorphismbased-dna-marker-for-fall-armyworm-lepidoptera-noctuidae-biotyping-a-case-study-from-the-fall-armyworm-outbreak-in-sri-lanka/3FA787706686A425402C86943906AF05#sec0
Last checked: 12/18/2020
Notes: Abstract only |
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| Abstract/Contents: | "Crop damage caused by fall armyworm (FAW), Spodoptera frugipera J.E. Smith (1797) (Lepidoptera: Noctuidae), has generated concern among agriculturists globally. In 2019, FAW was first reported in Sri Lanka, where it caused significant losses to corn crops. However, given that the two FAW biotypes - rice strain (R-FAW) and corn strain (C-FAW) - are morphologically identical, the biotype(s) present in Sri Lanka were unknown. The current study used the mitochondrial cytochrome c oxidase subunit I gene (mt-CO1) of FAW to biotype nine FAW samples collected in Sri Lanka. The resulting molecular phylogeny revealed that both R-FAW and C-FAW biotypes were present among the samples. In addition, we used the temperature-switch polymerase chain reaction (PCR) technique to develop a gel-based molecular marker. Two fragments were successfully amplified by the newly developed marker ABUOP0002, with fragment sizes of 341 bp from R-FAW and 204 bp from C-FAW. This demonstrates that ABUOP0002 can serve as a diagnostic gel-based molecular marker to identify the R-FAW and C-FAW biotypes from samples already identified to species level as S. frugiperda through taxonomical keys and provides a possible alternative to more expensive sequencing-based assays." |
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| Language: | English |
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| References: | 39 |
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| Note: | Pictures, b&w
Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Nanayakkara, D., D. Jayatilake, and S. Kodithuwakku. 2020. Development of a single nucleotide polymorphism-based DNA marker for fall armyworm (Lepidoptera: Noctuidae) biotyping: A case study from the fall armyworm outbreak in Sri Lanka. Can. Entomol. 152:p. 762-771. |
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| DOI: 10.4039/tce.2020.52 |
| Web URL(s):
https://www-cambridge-org.proxy1.cl.msu.edu/core/journals//canadian-entomologist/article/development-of-a-single-nucleotide-polymorphismbased-dna-marker-for-fall-armyworm-lepidoptera-noctuidae-biotyping-a-case-study-from-the-fall-armyworm-outbreak-in-sri-lanka/3FA787706686A425402C86943906AF05
Last checked: 12/18/2020
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Access conditions: Item is within a limited-access website
https://www-cambridge-org.proxy1.cl.msu.edu/core/services/aop-cambridge-core/content/view/3FA787706686A425402C86943906AF05/S0008347X20000528a.pdf/development_of_a_single_nucleotide_polymorphismbased_dna_marker_for_fall_armyworm_lepidoptera_noctuidae_biotyping_a_case_study_from_the_fall_armyworm_outbreak_in_sri_lanka.pdf
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Access conditions: Item is within a limited-access website |
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