Full TGIF Record # 317131
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Web URL(s):https://scisoc.confex.com/scisoc/2021am/meetingapp.cgi/Paper/133702
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Publication Type:
Content Type:Abstract or Summary only
Author(s):McElroy, J. Scott; Bi, Bo; Harris, James; Rutland, Claudia Ann; Porri, Aimone; Spesard, Bruce
Author Affiliation:McElroy: Auburn University, Auburn, AL; Bi and Rutland: Deprtment of Crop, Soil, and Environ. Sci., Auburn University, Auburn, AL; Harris: Auburn University, AL; Porri: Global Research and Development Agricultural Solutions, BASF, Limburgerhof, Germany; Spesard: Crop Science Division, Bayer R&D Services, Cary, NC
Title:Further identification of oxadiazon resistance in goosegrass (Eleusine indica) and improved understanding of the resistance mechanism
Section:Molecular techniques, genetics, and turfgrass breeding oral (includes student competition)
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C05 turfgrass science
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Meeting Info.:Salt Lake City, Utah: November 7-10, 2021
Source:ASA, CSSA and SSSA International Annual Meetings. 2021, p. 133702.
Publishing Information:[Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America]
# of Pages:1
Abstract/Contents:"Goosegrass (Eleusine indica L.) is one of the most problematic weeds in turfgrass and cropping systems. Oxadiazon, a protoporphyrinogen oxidase (PPO) inhibiting herbicides, is an effective preemergence treatment for goosegrass control and eventual resistance has developed. An A212T substitution in PPO1 was recently identified as a causal mechanism of oxadiazon resistance in goosegrass. Research was conducted to evaluate new populations for resistance to oxadiazon, determine if resistance can be detected using chlorophyll florescence, and develop a dCAPs assay for detecting the A212T mutation. 21 new suspected resistant populations were submitted to the Herbicide Resistance Diagnostic Lab at Auburn University with suspected resistance to oxadiazon. Plants treated with oxadiazon 1.12 kg ha-1 were rated on a binary scale of resistant (R) or susceptible (S) in comparison to previously diagnosed R and S biotypes. Nine of the 21 suspected R biotypes were diagnosed as R based on postemergence screen all of which encoded a nonsynonymous SNP resulting in A212T amino acid substitution in PPO1. A dCAPs primer effectively detected the A212T mutation of all populations containing the mutation without sequencing. Fv/Fm was evaluated as a diagnostic tool for fast detecting oxadiazon resistance. I50 values of Fv/Fm at 72 h after treatment were 0.62, 9.15 and 3.33 kg ha-1 oxadiazon for PBU (known susceptible), RB (known resistant) and Currituck (suspected resistant), respectively, indicating the newly diagnosed resistant population Currituck could be diagnosed with chlorophyll florescence; however, unknown variation exists between the two resistant populations. No other reported mutations occurred either in PPO1 or PPO2 of any populations. The target-site mutation A212T in PPO1 have been further substantiated as the primary mechanism of oxadiazon resistance."
Note:This item is an abstract only!
Includes video, "Further identification of oxadiazon resistance in goosegrass (Eleusine indica) and improved understanding of the resistance mechanism", 13:30
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
McElroy, J. S., B. Bi, J. Harris, C. A. Rutland, A. Porri, and B. Spesard. 2021. Further identification of oxadiazon resistance in goosegrass (Eleusine indica) and improved understanding of the resistance mechanism. Agron. Abr. p. 133702.
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