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| Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1994Articles/phyto84n10_1061.pdf#page=21
Last checked: 10/16/2015
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Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Kobayashi, D. |
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| Author Affiliation: | Dept. of Plant Pathology, Rutgers University, New Brunswick, NJ |
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| Title: | Characterization of a chitinase gene cloned from Xanthomonas maltophilla isolate 34S1 |
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| Meeting Info.: | 1994 APS Annual Meeting, August 6-10, 1994, Albuquerque, New Mexico |
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| Source: | Phytopathology. Vol. 84, No. 10, October 1994, p. 1081. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Summer patch; Magnaporthe poae; Chiten; Biological control
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| Abstract/Contents: | "Xanthomonas maltophilia isolate 34S1 was previously identified as a biocontrol agent of summer patch disease, caused by Magnaporthe poae. X. maltophilia 34S1, as well as all other X. maltophilia isolates screened, tested positive for chitinolytic activity as detected by clearing zones in agar media supplemented with collodial chitin. A genomic library of X. maltophilia 34S1 was constructed in the cosmid vector pLAFR3 and screened for chitinase activity. No cosmid clones that expressed chitinase activity were identified when the library was screened in E. coli. Two overlapping library clones, however, were found to express chitinase activity when the library was mobilized into Pseudonomas cepacia. The chitinase gene was subcloned to a 3 kb XhoI-SacI fragment, in which expression of activity was observed only in P. cepacia and certain other selected Pseudonomas strains. Weak expression of chitinase activity could be observed in E. coli that contained the gene positioned behind the lac promoter, but only after prolonged incubations of several days, suggesting a problem with secretion of the protein." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Kobayashi, D. 1994. Characterization of a chitinase gene cloned from Xanthomonas maltophilla isolate 34S1. Phytopathology. 84(10):p. 1081. |
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| Web URL(s):
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1994Articles/phyto84n10_1061.pdf#page=21
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 599 .P48 |
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