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Web URL(s): | https://scisoc.confex.com/scisoc/2022am/meetingapp.cgi/Paper/142516 Last checked: 01/25/2023 Requires: JavaScript; HTML5 |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Cors, Jonathan;
Watkins, Eric;
Smanski, Michael |
Author Affiliation: | Cors: Presenting Author and University of Minnesota - Twin Cities; Watkins: University of Minnesota - Twin Cities; Smanski: University of Minnesota |
Title: | Reducing environmental risk in genetically modified perennial ryegrass |
Section: | Turfgrass Physiology, Molecular Biology, Microbiome, and Genetics Poster (includes student competition) Other records with the "Turfgrass Physiology, Molecular Biology, Microbiome, and Genetics Poster (includes student competition)" Section
C05 turfgrass science Other records with the "C05 turfgrass science" Section
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Meeting Info.: | Baltimore, Maryland: November 6-9, 2022 |
Source: | ASA, CSSA, SSSA International Annual Meeting. 2022, p. 142516. |
Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America] |
# of Pages: | 1 |
Abstract/Contents: | "Genetically modified turfgrasses can incorporate several unique genes that can increase profits through different pathways than traditional plant breeding methods. Specifically, perennial ryegrass, an economically important species of turfgrass, would greatly benefit from the ability to be genetically modified. To safely release a genetically modified turfgrass, it is first necessary to address the high potential for that genetic edit to transfer to wild type populations through hybridization. Engineered genetic incompatibility (EGI), is a way to enact a species like barrier to reproduction, cutting off gene flow with wild varieties. EGI utilizes a CRISPR-activator system with a gRNA targeting the wild type promoter of a tightly controlled gene, while at the same time having a silent point mutation within the EGI plant at that same promoter. Within the EGI plant, the CRISPR-activator doesnt bind, as the promoter region has been mutated, and there is no effect. However, once it crosses with a wild type organism, it will have one copy of the wild type promoter. This gives the CRISPR-activator a place to bind, driving overexpression and consequently death of the hybrid offspring. While this effectively makes it impossible for an EGI plant to cross with wild type individuals, it does not mean that an EGI plant is sterile, in contrast to other biocontainment methods. EGI plants can still be bred with other plants that have that same EGI mutation, producing viable offspring. This biocontrol method would allow for the further introgression of other beneficial genetic mutations without the concern for uncontrolled spread of said genetic mutation." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! "372" |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Cors, J., E. Watkins, and M. Smanski. 2022. Reducing environmental risk in genetically modified perennial ryegrass. Agron. Abr. p. 142516. |
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