Full TGIF Record # 325009
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Web URL(s):https://scisoc.confex.com/scisoc/2022am/meetingapp.cgi/Paper/145641
    Last checked: 01/31/2023
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Publication Type:
Content Type:Abstract or Summary only
Author(s):Diehl, Katherine H.; Elmore, Matthew T.; Patterson, Eric
Author Affiliation:Diehl: Presenting Author; Elmore: Rutgers University; Patterson: Michigan State University
Title:TAU1 alpha tublin missense mutation found in several goosegrass (Eleusine indica) populations in New Jersy
Section:Turfgrass pest management oral I (includes student competition)
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C05 turfgrass science
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Meeting Info.:Baltimore, Maryland: November 6-9, 2022
Source:ASA, CSSA, SSSA International Annual Meeting. 2022, p. 145641.
Publishing Information:[Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America]
# of Pages:1
Abstract/Contents:"This objective of this research was to investigate herbicide resistance of multiple putative goosegrass biotypes across NJ and elucidate possible mechanisms of resistance within each biotype. We hypothesized that resistance to mitotic herbicides (dithiopyr and prodiamine) is underreported in cool-season turfgrass and potentially widespread in New Jersey, following decades on reliance on preemergence herbicides for goosegrass and crabgrass control on golf courses. Plants from three putative-resistant biotypes (JB, CV, WL; all NJ golf courses), and two putative-susceptible biotypes (SK and DD; a home lawn and tree farm) were harvested and propagated in a greenhouse for seed. In 2021, dose-response experiments were conducted for all biotypes in 10 by 10 cm pots of 80:20 sand/peat (v/v), biotype were also included. Prodiamine and dithiopyr (0, 50, 100, 500 and 5000 g/ha) treatments were applied to all biotypes within 24 hr of seeding, and a known dithiopyr-resistant (PB-R) and known-susceptible (HF-S) biotype was included for control. Plant count data and above ground biomass was collected 28 DAT. Four plants from each biotype were randomly selected from >100 plants grown from seed for genetic sequencing of the a-tubulin region associated with TSR. Biotype sequences did not different between replicates and were subjected to multiple sequence alignment across biotypes in comparison to R/S references (AJ005598-9). Seedling emergence data from dose response experiments were subjected to nonlinear regression in GraphPad Prism, to generate IC50 values used to determine levels of resistance for each biotype. Herbicide resistance was confirmed for suspect biotypes. Two mutations were identified, but mutant phenotypes varied by biotype. Dithiopyr resistant PB-R and JB (IC50 = 34, 121) and susceptible (SK, DD, IC50 = <10 g/ha) biotypes shared Leu-136-Phe mutations. Prodiamine-resistant WL and CV biotypes (IC50 = 80 and 175) differed significantly in their tolerance to dithiopyr, but shared Thr-239-Ile mutations."
This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Diehl, K. H., M. T. Elmore, and E. Patterson. 2022. TAU1 alpha tublin missense mutation found in several goosegrass (Eleusine indica) populations in New Jersy. Agron. Abr. p. 145641.
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    Last checked: 01/31/2023
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