Full TGIF Record # 32976
Item 1 of 1
Publication Type:
i
Refereed
Author(s):Mysore, Kirankumar S.; Baird, WM. Vance
Author Affiliation:Grad. Res. Asst. and Assoc. Prof., Dept. Hortic., Clemson University, Poole Agric. Bldg., Clemson, SC
Title:Molecular characterization of the tubulin-related gene families in herbicide resistant and susceptible goosegrass (Eleusine indica)
Source:Weed Science. Vol. 43, No. 1, January-March 1995, p. 28-33.
Publishing Information:Champaign, IL: Weed Science Society of America
# of Pages:6
Keywords:TIC Keywords: Eleusine indica; Dinitramine; Resistance; Genes; Restriction fragment length polymorphism; Phenotypes; DNA
Abstract/Contents:"Goosegrass, widespread throughout the tropics and subtropics, is one of the most noxious weeds known. Recently, biotypes of goosegrass have been found resistant to the dinitroaniline herbicides. An alteration in the structure/composition of a tubulin protein has been postulated as an explanation for the hyperstable microtubules and the resistant phenotype. Our study was initiated to investigate the structure of the alpha-, beta- and gamma-tubulin related gene sequence in resistant, intermediately resistant, and susceptible biotypes. Heterologous tubulin gene clones were used as probes of restriction endonuclease-digested genomic DNA from each biotype, to determine gene size and copy number and to screen for restriction fragment length polymorphisms. The tubulin genes are organized into gene families. There are three to five alpha-tubulin genes, four to seven beta-tubulin genes, and four to eight gamma-tubulin genes. There was no evidence of multiple copies or tandem repeats of any individual gene sequence. Although RFLPs were observed, no significant difference between the herbicide-response phenotypes can be attributed to large deletions or insertions in a tubulin gene."
Language:English
References:42
Note:Pictures, b/w
Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Mysore, K. S., and W. V. Baird. 1995. Molecular characterization of the tubulin-related gene families in herbicide resistant and susceptible goosegrass (Eleusine indica). Weed Sci. 43(1):p. 28-33.
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