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DOI: | 10.1139/_|n_Pending_DOI_Completion |
Web URL(s): | http://www.nrcresearchpress.com/loi/cjb Last checked: 01/14/2016 Notes: Pending URL completion |
Publication Type:
| Refereed |
Author(s): | Chen, P. M.;
Gusta, L. V. |
Author Affiliation: | Crop Development Centre, University of Saskatchewan, Saskatoon, Sask., Canada |
Title: | Cold acclimation of wheat and smooth brome-grass cell suspensions |
Source: | Botany [Canada]. 1982, p. 1207-1211. |
Publishing Information: | Vancouver, British Columbia, Canada: The National Research Council of Canada |
# of Pages: | 5 |
Keywords: | TIC Keywords: Bromus inermis; Cell culture; Cold resistance; Triticum; Bromus; Temperatures; Freezing techniques
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Abstract/Contents: | "Cell suspension cultures derived from spring wheat cv. Manitou (Triticum aestivum) and winter wheat cv. Kharkov tolerated freezing on agar media to -15 °C and -19 °C, respectively, after 10 days of acclimation at 4 °C without light. Cell water content decreased from 14 g H2O/g dry matter to 11 g H2O/g dry matter for both cultures during the hardening period. Cell viability following freezing was determined by both fluorescein diacetate vital-staining and regrowth procedures. The vital-staining technique was much quicker, simpler, and as accurate as the regrowth test. Cell suspension cultures derived from smooth brome-grass (Bromus inermis) readily acclimate to -20 °C either on agar media or as a cell suspension. Brome-grass cells washed prior to freezing with nutrient media minus sucrose were less hardy than cells washed with the nutrient media plus sucrose." |
Language: | English |
References: | 15 |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Chen, P. M., and L. V. Gusta. 1982. Cold acclimation of wheat and smooth brome-grass cell suspensions. Can. J. Bot. p. 1207-1211. |
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| DOI: 10.1139/_|n_Pending_DOI_Completion |
| Web URL(s): http://www.nrcresearchpress.com/loi/cjb Last checked: 01/14/2016 Notes: Pending URL completion |
| MSU catalog number: b2201022a |
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