Full TGIF Record # 34261
Item 1 of 1
Publication Type:
i
Report
Author(s):Steiner, A. M.; Goeritz, A.
Author Affiliation:Institut für Pflanzenz^D:uchtung, Saatgutforschung ung Populationsgenetik 350, Universität Hohenheim, 7000 Stuttgart 70
Title:Die Unterscheidung des Saatguts von Sorten der Lägerrispe (Poa supina Schrad.) und von Järige Rispe (Poa annua L.) mittels Elektrophorese der Speicherproteine
Translated Title:The differentiation of seed of cultivars of Poa supina Schrad. and of Poa annua L. by the electrophoresis of storage proteins
Source:Rasen-Turf-Gazon. Vol. 22, No. 4, December 1991, p. 90-94.
Publishing Information:Bonn-Bad Godesberg, Germany: Hortus Verlag
# of Pages:5
Keywords:TIC Keywords: SDS-PAGE; Poa supina; Poa annua; Analytical methods
Abstract/Contents:Describes:Test performed by using electrophoresis to analyize the storage proteins samples of Poa supina and Poa annua for purposes of identification. The storage proteins can be obtained directly from the florets, while Isoenzymes can only be obtained from seedlings. Electrophoresis is therefore far less costly, and proves to be a more effective means of cultivar identification. Coloring the protein maps, all varieties of Poa supina tested (supranova, petto, posipa, and supra) showed at least one band that no sample of Poa annua possessed. The converse applied when testing Poa annua. "In bulk samples admixtures of Poa annua in Poa supina are detectable from about 5% upwards." Single seed analysis allows for tests to be done on cultivar purity, based on the characteristic bands that each variety show. Supranova proved to have bands with the fewest variations, allowing its purity to most easily be acertained. The other cultivars had too many variants in their bands to make any accurate purity analysis possible. By augmenting the testing methods used in this stusy though, a more comprehensive differentiation of cultivars using electrophoresis should prove possible.
Language:German
References:19
Note:Pictures, b/w
Abstract appears in German, English, and French
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Stiener, A. M., and A. Goeritz. 1991. Die Unterscheidung des Saatguts von Sorten der Lägerrispe (Poa supina Schrad.) und von Järige Rispe (Poa annua L.) mittels Elektrophorese der Speicherproteine. (In German) Rasen Turf Gazon. 22(4):p. 90-94.
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