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| DOI: | 10.21273/HORTSCI.29.5.515c |
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| Web URL(s): | https://journals.ashs.org/hortsci/view/journals/hortsci/29/5/article-p515c.xml?rskey=vLwUW9
Last checked: 11/13/2019
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Ke, Shanqiang;
Lee, Chiwon W. |
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| Author Affiliation: | Department of Crop and Weed Sciences, North Dakota Stae University |
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| Title: | Plant regeneration from embryogenic callus tissues of Kentucky bluegrass |
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| Meeting Info.: | 91st Annual Meeting of ASHS, 7-10 August, 1994, Corvallis, OR |
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| Source: | HortScience. Vol. 29, No. 5, May 1994, p. 515. |
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| Publishing Information: | Alexandria, VA: American Society for Horticultural Science |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Regeneration; Calli; Embryogenesis; Poa pratensis
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| Abstract/Contents: | "Coleoptile tissues from dark-germinated seedlings of Kentucky bluegrass (Poa pratensis L.) cv. Touchdown were excised and cultured on MS medium supplemented with 1.5 - 2.5 mg/liter picloram plus 0.2 mg/liter benzyladenine (BA) or with 4 mg/liter 2, 4-D. Embryogenic calli were formed on media containing 1.5 mg/liter picloram plus 2.5 mg/liter 2, 4-D in the dark. When these embryogenic calli were subcultured on MS medium containing either 0.15 - 0.3 mg/liter picloram or 0.2 - 0.5 mg/liter 2, 4-D in a 16-h day/8-h night photoperiod, 10.5% of the cultures regenerated shoots. Pretreatment of cultures in the dark for 2 weeks prior to light exposure slightly increased the plant regeneration efficiency to 15.5%. Pigmentation of the regenerants varied with a ratio of 8.5 completely green: 2.5 green plus albino: 1 completely albino plants. The shoots were multiplied in the medium containing 0.5mg/liter BA plus either 0.2 mg/liter picloram or 0.1 mg/liter indoleacetic acid (IAA). Over 90% cultures in the shoot proliferation medium produced roots after 4 weeks." |
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| Language: | English |
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| References: | 0 |
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| Note: | Abstract #582
This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Ke, S., and C. W. Lee. 1994. Plant regeneration from embryogenic callus tissues of Kentucky bluegrass. HortScience. 29(5):p. 515. |
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| DOI: 10.21273/HORTSCI.29.5.515c |
| Web URL(s):
https://journals.ashs.org/hortsci/view/journals/hortsci/29/5/article-p515c.xml?rskey=vLwUW9
Last checked: 11/13/2019
Requires: PDF Reader |
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MSU catalog number: SB 1 .H64 |
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