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| DOI: | 10.21273/HORTSCI.29.5.515f |
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| Web URL(s): | https://journals.ashs.org/hortsci/view/journals/hortsci/29/5/article-p515f.xml?rskey=Y4OkoP
Last checked: 11/13/2019
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Griffin, Jeffrey D.;
Dibble, Margaret S. |
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| Author Affiliation: | Dept. of Plant, Soil, and Entomological Sciences, University of Idaho |
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| Title: | Improved regeneration frequency of Kentucky bluegrass from callus cultures |
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| Meeting Info.: | 91st Annual Meeting of ASHS, 7-10 August, 1994, Corvallis, OR |
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| Source: | HortScience. Vol. 29, No. 5, May 1994, p. 515. |
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| Publishing Information: | Alexandria, VA: American Society for Horticultural Science |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Regeneration; Calli; Poa pratensis
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| Abstract/Contents: | "Genetic transformation of Kentucky bluegrass (Poa pratensis L.) requires methods for high-frequency regeneration of plantlets from cultural cells. Regeneration of this important turf species has been reported, albeit at a low frequency from seed-derived callus. We tested the potential of 3 synthetic auxins, used in the callus initiation and growth medium, for their ability to promote regeneration in 3 bluegrass varieties. 10 μM 2,4-D promoted regeneration from 0 to 5% of calli, 30μM and 60μM picloram promoted regeneration from 0 to 8% of all calli, and 10 to 60μM dicamba, in combination with BA, promoted regeneration from 1% to 8% of calli. In a subsequent experiment, both 60μM and 90μM dicamba, with 20μ BA, promoted regeneration from 45% of calli averaged across varieties. These media were tested for the promotion of regeneration in 12 diverse bluegrass varieties. Although up to 45% of the calli from some varieties regenerated plantlets, the response of other varieties was markedly lower, indicating a genetic component in the response to these media." |
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| Language: | English |
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| References: | 0 |
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| Note: | Abstract #585
This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Griffin, J. D., and M. S. Dibble. 1994. Improved regeneration frequency of Kentucky bluegrass from callus cultures. HortScience. 29(5):p. 515. |
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| DOI: 10.21273/HORTSCI.29.5.515f |
| Web URL(s):
https://journals.ashs.org/hortsci/view/journals/hortsci/29/5/article-p515f.xml?rskey=Y4OkoP
Last checked: 11/13/2019
Requires: PDF Reader |
| 
MSU catalog number: SB 1 .H64 |
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