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Web URL(s): | https://journals.ashs.org/hortsci/downloadpdf/journals/hortsci/30/6/article-p1260.xml Last checked: 11/13/2019 Requires: PDF Reader Notes: Direct Download |
Publication Type:
| Refereed |
Author(s): | Zhang, Mei;
Dernoeden, Peter H. |
Author Affiliation: | Department of Agronomy, University of Maryland, College Park, MD 20742 |
Title: | Facilitating anastomosis grouping of Rhisoctonia solani isolates from cool-season turfgrasses |
Source: | HortScience. Vol. 30, No. 6, October 1995, p. 1260-1262. |
Publishing Information: | Alexandria, VA: American Society for Horticultural Science |
# of Pages: | 3 |
Keywords: | TIC Keywords: Rhizoctonia solani; Disease identification; Methodology; Analytical methods; Agrostis; Brown patch; Lolium perenne; Festuca arundinacea
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Abstract/Contents: | "Determining anastomosis groups (AGs) of Rhizoctonia solani Kühn isolates is tedious and time-consuming. Three previously described methods (i.e., cellophane strip, glass slide, petri dish) were compared to determine which was the most rapid and accurate. Colony characteristics also were assessed to tentatively identify AGs. All techniques were accurate. The cellophane strip method was most time-consuming, and the time required for hyphal overlap with the glass slide method was not generally predictable. Pairing isolates in a petri dish containing a thin layer of water agar was reliable and was the simplest technique. There was little variation in colony pigmentation or sclerotia color, shape, or formation patterns within AG-1 IA (n=34), AG-2-2 IIIB (n=46), and AG-4 (n=5); the former two Ags are the ones most commonly isolated from cool-season turfgrasses. Accordingly, R. solani isolates from turfgrasses may be assigned tentatively to an AG based on colony pigmentation and sclerotial characteristics." |
Language: | English |
References: | 22 |
Note: | Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Zhang, M., and P. H. Dernoeden. 1995. Facilitating anastomosis grouping of Rhisoctonia solani isolates from cool-season turfgrasses. HortScience. 30(6):p. 1260-1262. |
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| MSU catalog number: SB 1 .H64 |
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