| |
| Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1995Articles/phyto85n12_1554.pdf#page=4
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
|---|
| Publication Type:
| Report |
|---|
| Content Type: | Abstract or Summary only |
|---|
| Author(s): | Holtman, M. A.;
Kobayashi, D. |
|---|
| Author Affiliation: | Rutgers University, Dept. Plant Pathology, New Brunswick, NJ 08903 |
|---|
| Title: | Cloning of ^D^*B-1,3-glucanases from the bacterial biocontrol agent, N4-7 |
|---|
| Section: | APS Northeastern Division
Other records with the "APS Northeastern Division" Section
|
|---|
| Meeting Info.: | October 18-20, 1995, Quebec, Canada |
|---|
| Source: | Phytopathology. Vol. 85, No. 12, December 1995, p. 1557. |
|---|
| Publishing Information: | St. Paul, MN: American Phytopathological Society |
|---|
| # of Pages: | 1 |
|---|
| Keywords: | TIC Keywords: Biological control; Summer patch; Magnaporthe poae; Enzyme activity; Clones
|
|---|
| Abstract/Contents: | "N4- 7, a gram-negative soil bacterium, is an effective biocontrol agent of summer patch disease of Kentucky bluegrass caused by Magnaporthe poae, N4-7 is unique in that it produces all common degradative enzymes such as chitinase, glucanase, lipase and protease. Six cosmid clones which produce ^D^*B-1,3-glucanase activities were isolated from the wildtype genomic library expressed in an Enterobacter sp. The clones shared several common restriction fragments but were varied in substrate specificity for laminarin (soluble ^D^*B-1,3-glucan), zymosan (insoluble ^D^*B-1,3-glucan) and 4-methylumbelliferyl -^D^*B-glucoside. Two clones were active against all three substrates and contained both 3 and 8 kb BamHI fragments. Preliminary mapping suggests that the 3 kb region is responsible for laminarinase activity whereas the linked 8 kb region confers ^D^*B-glucosidase and zymosanase activity." |
|---|
| Language: | English |
|---|
| References: | 0 |
|---|
| Note: | This item is an abstract only! |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Holtman, M. A., and D. Kobayashi. 1995. Cloning of ^D^*B-1,3-glucanases from the bacterial biocontrol agent, N4-7. Phytopathology. 85(12):p. 1557. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=36905 |
| If there are problems with this record, send us feedback about record 36905. |
| Choices for finding the above item: |
|---|
| Web URL(s):
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1995Articles/phyto85n12_1554.pdf#page=4
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: SB 599 .P48 |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
