Full TGIF Record # 37363
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Web URL(s):http://www.tandfonline.com/doi/abs/10.1300/J099v01n03_01#.UqnHveKQO8E
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Publication Type:
i
Report
Author(s):Bell, G. E.; McDonald, M. B. Jr.; Danneberger, T. K.
Author Affiliation:Department of Agronomy, The Ohio State University, Columbus, OH 43210
Title:Electrophoretic evaluation of esterase isozymes from turfgrass seed blends and mixtures
Source:Journal of Turfgrass Management. Vol. 1, No. 3, 1995, p. 1-11.
# of Pages:11
Publishing Information:Binghamton, NY: Food Products Press (Haworth Press)
Keywords:TIC Keywords: Esterase isoenzymes; Seed mixtures; Cultivars; Poa pratensis; pH; Fine fescues; Analytical methods; Evaluative methods; Seed testing
Abstract/Contents:"Turfgrass seeds are commonly blended to improve performance. However, once physically blended, no fast, reliable technique is available to identify the cultivars and the proportion of each cultivar in the blend. The objective of this study was to isolate specific species and cultivars in seed blends or mixtures through examination of esterase banding patterns on electrophoretic gels, and to compare band intensities unique to each cultivar or species when blended in increasing or decreasing proportions. Two cultivars of Kentucky bluegrass (Poa pratensis L.) were blended by weight in proportions ranging from 0 to 100%. Protein extracted, separated on isoelectric focusing gels (pH gradient 3 to 9) and stained for esterase activity. Mixtures of the fine fescue (Festuca spp.) species only and mixtures of both fine fescue species and Kentucky bluegrass cultivars were prepared in the same manner. Gels were visually analyzed for the presence of characteristic bands unique to each cultivar or species and for intensities of characteristic bands when cultivar proportions were varied within a seed blend or mixture. The results indicated that visual discriminations could be made between cultivars and species in turfgrass blends or mixtures and that bands unique to a cultivar or species varied in intensity with their concentration in a blend or mixture. These findings have importance to seed companies and comsumers interested in monitoring the composition of seed blends and mixtures subsequent to physical mixing."
Language:English
References:11
Note:Figures
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Bell, G. E., M. B. Jr. McDonald, and T. K. Danneberger. 1995. Electrophoretic evaluation of esterase isozymes from turfgrass seed blends and mixtures. J. Turfgrass Manage. 1(3):p. 1-11.
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Web URL(s):
http://www.tandfonline.com/doi/abs/10.1300/J099v01n03_01#.UqnHveKQO8E
    Last checked: Item not verified
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
    Notes: Abstract and Guide page only
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MSU catalog number: SB 433 .J68
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