Full TGIF Record # 37951
Item 1 of 1
Publication Type:
i
Proceedings
Author(s):Elliott, Monica L.
Author Affiliation:University of Florida, IFAS, Ft Lauderdale Research & Education Center
Title:Detection of gaeumannomyces fungi with molecular techniques
Meeting Info.:Orlando, FL: October 16-19, 1977
Source:Proceedings of the Florida Turfgrass Conference. Vol. 39, 1991, p. 17-18.
Publishing Information:Orlando, FL: Florida Turfgrass Association
# of Pages:2
Keywords:TIC Keywords: Cynodon; Gaeumannomyces; DNA; Pythium diseases; Fungi; Polymerase chain reaction; Techniques
Abstract/Contents:"Bermudagrass decline is a destructive root rot disease of hybrid bermudagrass used for golf course putting greens in Florida. It has recently been determined that G. graminus var. graminis is a causal agent of bermudagrass decline...The role of G. incrustans or Phialophora spp. in the disease complex has yet to be confirmed. One of the primary problems associated with Gaeumannomyces group of fungi is detection. The selective medium currently used...is an improvement over standard, non-selective media, but it is not fool-proof and positive results are very slow in developing. As a general rule, a minimum of 7 days is required before the distinctive growth of Gaeumannomyces appears on the medium. During that time, the potential for contamination by saprophytes is high. A polymerase chain reaction (PCR) technique for detection of G. graminis...has recently been developed. In theory, PCR requires that only one copy of a particular piece of DNA be present in, for example, a piece of root tissue. DNA is the molecular blueprint for all life forms. Via a series of reactions, the single DNA copy is amplified to a point where it can be easily observed on an agarose gel. The primary objective of this study is to learn this technique and apply it to the bermudagrass decline problem. Since the Gaeumannomyces group of fungi are also implicated as causal agents of root rot in St Augustinegrass, centipedegrass and zoysiagrass, the technique will aid in the study of these disease problems also."
Language:English
References:3
See Also:Other items relating to: BERDEC
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Elliott, M. L. 1991. Detection of gaeumannomyces fungi with molecular techniques. p. 17-18. In Proceedings of the Florida Turfgrass Conference. Orlando, FL: October 16-19, 1977. Orlando, FL: Florida Turfgrass Association.
Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=37951
If there are problems with this record, send us feedback about record 37951.
Choices for finding the above item:
Find Item @ MSU
MSU catalog number: SB 433 .F53
Request through your local library's inter-library loan service (bring or send a copy of this TGIF record)