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| Web URL(s): | http://www.sciencedirect.com/science/article/pii/0098847280902336/pdf?md5=2a4d8a734805b47533350887861747f3&pid=1-s2.0-0098847280902336-main.pdf
Last checked: 10/02/2015
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| Author(s): | Conger, B. V.;
Lowe, K. W.;
Carabia, J. V. |
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| Author Affiliation: | Dep. of Pl. and Soil Sci., Tennessee Univ., Knoxville, TN 37916, USA |
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| Title: | Relative DNA content of cells in Festuca arundinacea and Dactylis glomerata calli of different ages |
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| Source: | Environmental and Experimental Botany. Vol. 20, No. 4, 1980, p. 401-408. |
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| # of Pages: | 8 |
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| Keywords: | TIC Keywords: Festuca arundinacea; Dactylis glomerata; Tissue culture; Festuca; Dactylis; Metabolism; Nucleic acid; DNA; Feulgen staining
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| Abstract/Contents: | Changes in nuclear DNA content in cells of tall fescue cv. Ky-31 and cocksfoot cv. Potomac were examined in embryo-derived calluses ranging from 3 to 24 wk old. Calluses were induced and maintained on a modified Murashige-Skoog medium containing 22.6 mu M 2,4-D. Calluses were divided and one piece was fixed in 3:1 ethanol:acetic acid and the other transferred to fresh maintenance medium at 3, 6, 9, 12, 18 and 24 wk after initial plating of mature embryos. Fixed calluses were processed through a cold hydrolysis technique. Feulgen stained callus pieces were squashed in 45% acetic acid and relative DNA contents were measured with a microscope cytophotometer. Results showed predominately 2C nuclei in calluses of both spp. regardless of callus age. More cells with high DNA contents ( more than or equal to 4C) were found in cocksfoot than tall fescue calluses. The proportion of 2C cells increased with increasing callus age, especially in tall fescue. Cells of various sizes and shapes were observed in calluses of both spp. and very large cells with small (2C) nuclei were common in callus tissue of all ages. The mitotic index was low and decreased with increasing callus age especially in tall fescue. |
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| Language: | English |
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| References: | 23 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Conger, B. V., K. W. Lowe, and J. V. Carabia. 1980. Relative DNA content of cells in Festuca arundinacea and Dactylis glomerata calli of different ages. Environmental and Experimental Botany. 20(4):p. 401-408. |
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| Web URL(s):
http://www.sciencedirect.com/science/article/pii/0098847280902336/pdf?md5=2a4d8a734805b47533350887861747f3&pid=1-s2.0-0098847280902336-main.pdf
Last checked: 10/02/2015
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: QK 711 .A1 R3 |
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