Full TGIF Record # 39594
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Author(s):Freeman, Glenn W.; Wagg, Charles A.; Mileva, Mayia M.
Author Affiliation:Division of Plant Industry, New Jersey Department of Agriculture, CN 330, Trenton, NJ 08625 USA
Title:Identification of turfgrass cultivars using reversed-phase high-performance liquid chromatography
Source:Seed Science and Technology. Vol. 24, No. 3, 1996, p. 495-504.
# of Pages:10
Publishing Information:International Seed Testing Association
Keywords:TIC Keywords: HPLC; Chromatography; Cultivar identification; Festuca arundinacea; Poa pratensis; Agrostis stolonifera
Abstract/Contents:"Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) of cereal grain proteins has received much attention in the literature in recent years as a quick and efficient means of cultivar identification. A study of tall fescue (Festuca arundinacea Schreber), Kentucky bluegrass (Poa pratensis L.), and creeping bentgrass (Agrostis stolonifera var. palustris (Hudson) Farwell) was undertaken to determine if RP-HPLC could be adapted to the cultivar identification of turfgrass species. Chromatograms of different cultivars of these species were all different and were used to characterize those cultivars. RP-HPLC was shown to be a quick, repeatable and reliable method of turfgrass cultivar identification for general screening of seed lots. Due to the detection sensitivity of the apparatus used and the technical problems of working with small volumes, the analysis of single seeds for varietal identity was not possible. Mixtures and blends of seeds were analyzed and compared. The sensitivity of the detector was such that the changes of area under the peaks of the chromatogram did not become obvious until proportions increased or decreased 20% in total composition."
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Freeman, G. W., C. A. Wagg, and M. M. Mileva. 1996. Identification of turfgrass cultivars using reversed-phase high-performance liquid chromatography. Seed Sci. Technol. 24(3):p. 495-504.
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