| |
| Web URL(s): | http://www.tandfonline.com/doi/abs/10.1300/J099v02n02_05
Last checked: Item not verified
Requires: PDF Reader
Access conditions: Item is within a limited-access website
Notes: Abstract and Guide page only |
|---|
| Publication Type:
| Report |
|---|
| Author(s): | Sugiura, Kiyoyuki;
Inokuma, Chie;
Imaizumi, Nobuyuki;
Cho, Chol |
|---|
| Author Affiliation: | Research Associates and Senior Research Scientists, Japan Turfgrass Inc. 3-6-2, Akanehama, Narashino-city Chiba 275, Japan |
|---|
| Title: | Transgenic creeping bentgrass (Agrostis palustris Huds.) plants regenerated from protoplasts |
|---|
| Source: | Journal of Turfgrass Management. Vol. 2, No. 2, 1997, p. 43-53. |
|---|
| Publishing Information: | Binghamton, NY: Food Products Press (Haworth Press) |
|---|
| # of Pages: | 11 |
|---|
| Keywords: | TIC Keywords: Agrostis stolonifera; Phosphotransferase; Electroporation; Genetic transformation; Genes
|
|---|
| Abstract/Contents: | "Electroporation was used to introduce hygromycin phosphotransferase gene into creeping bentgrass (Agrostis palustris Huds.) protoplasts isolated from embryogenic suspension cells. After the subsequent selections with 50 mg L ā»Ā¹ hygromycin, several plantlets were regenerated from hygromycin resistant calli. PCR and Southern hybridization analysis of randomly chosen plants indicated that the transgene was integrated into their genomes and no false positives were found. The results suggested that hygromycin phosphotransferase gene was effective selectable marker gene for the selection of transformed cells. Overall frequency for hygromycin resistant colony formation in this report was 1-3 x 10ā»ā¶ based on the number of treated protoplasts." |
|---|
| Language: | English |
|---|
| References: | 17 |
|---|
| Note: | Pictures, b/w
Figures |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Sugiura, K., C. Inokuma, N. Imaizumi, and C. Cho. 1997. Transgenic creeping bentgrass (Agrostis palustris Huds.) plants regenerated from protoplasts. J. Turfgrass Manage. 2(2):p. 43-53. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=40229 |
| If there are problems with this record, send us feedback about record 40229. |
| Choices for finding the above item: |
|---|
| Web URL(s):
http://www.tandfonline.com/doi/abs/10.1300/J099v02n02_05
Last checked: Item not verified
Requires: PDF Reader
Access conditions: Item is within a limited-access website
Notes: Abstract and Guide page only |
| 
MSU catalog number: SB 433 .J68 |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
