Full TGIF Record # 40229
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Web URL(s):http://www.tandfonline.com/doi/abs/10.1300/J099v02n02_05
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Publication Type:
i
Report
Author(s):Sugiura, Kiyoyuki; Inokuma, Chie; Imaizumi, Nobuyuki; Cho, Chol
Author Affiliation:Research Associates and Senior Research Scientists, Japan Turfgrass Inc. 3-6-2, Akanehama, Narashino-city Chiba 275, Japan
Title:Transgenic creeping bentgrass (Agrostis palustris Huds.) plants regenerated from protoplasts
Source:Journal of Turfgrass Management. Vol. 2, No. 2, 1997, p. 43-53.
# of Pages:11
Publishing Information:Binghamton, NY: Food Products Press (Haworth Press)
Keywords:TIC Keywords: Agrostis stolonifera; Phosphotransferase; Electroporation; Genetic transformation; Genes
Abstract/Contents:"Electroporation was used to introduce hygromycin phosphotransferase gene into creeping bentgrass (Agrostis palustris Huds.) protoplasts isolated from embryogenic suspension cells. After the subsequent selections with 50 mg L ⁻¹ hygromycin, several plantlets were regenerated from hygromycin resistant calli. PCR and Southern hybridization analysis of randomly chosen plants indicated that the transgene was integrated into their genomes and no false positives were found. The results suggested that hygromycin phosphotransferase gene was effective selectable marker gene for the selection of transformed cells. Overall frequency for hygromycin resistant colony formation in this report was 1-3 x 10⁻⁶ based on the number of treated protoplasts."
Language:English
References:17
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Figures
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Sugiura, K., C. Inokuma, N. Imaizumi, and C. Cho. 1997. Transgenic creeping bentgrass (Agrostis palustris Huds.) plants regenerated from protoplasts. J. Turfgrass Manage. 2(2):p. 43-53.
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Web URL(s):
http://www.tandfonline.com/doi/abs/10.1300/J099v02n02_05
    Last checked: Item not verified
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
    Notes: Abstract and Guide page only
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MSU catalog number: SB 433 .J68
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