Full TGIF Record # 40517
Item 1 of 1
Publication Type:
i
Refereed
Author(s):Macklon, A. E. S.; Grayston, S. J.; Shand, C. A.; Sim, A.; Sellars, S.; Ord, B. G.
Author Affiliation:Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH, UK
Title:Uptake and transport of phosphorus by Agrostis Capillaris seedlings from rapidly hyrolysed organic sources extracted from ³²P-labelled bacterial cultures
Source:Plant and Soil. Vol. 190, No. 1, March 1997, p. 163-167.
# of Pages:5
Publishing Information:Dordrecht, Netherlands: Kluwer Academic Publishers
Related Web URL:http://link.springer.com/article/10.1023/A%3A1004294617461
    Last checked: 10/14/2015
    Notes: Abstract only
Keywords:TIC Keywords: Uptake; Phosphates; Bacteria; Phosphorus; Agrostis tenuis
Abstract/Contents:"Cultures of the soil bacterium Serratia liquifaciens grimesii were grown with ³²P labelled phosphate, to produce a uniformly ³²P labelled source of microbial P. Extracts of the bacteria were prepared by sonication, dialysis and filtration to provide a clear sterile solution which was characterised in terms of dissolved organic and condensed P (DOP and DCP) and molecular weight range. The extract was used as a source of P to Agrostis capillaris L. seedlings in nutrient solution from which orthophosphate was omitted. In a time course experiment, root surface phosphatase activity increased as soon as extract was added to the root medium, DOP was rapidly hydrolysed and orthophosphate concentration increased rapidly. These processes were complete within about 8 h, after which phosphatase activity fell to its original level, and the plants absorbed molybdate reactive P from the nutrient solution so that it reached its original concentration over 48 h. DCP concentrations did not change significantly throughout the experiment. This work clearly demonstrated that DOP but not DCP, as a component of a bacterial extract produced by a relatively straightforward method, was quickly hydrolysed and the P made available for plant uptake."
Language:English
References:20
Note:Figures
Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Macklon, A. E. S., S. J. Grayston, C. A. Shand, A. Sim, S. Sellars, and B. G. Ord. 1997. Uptake and transport of phosphorus by Agrostis Capillaris seedlings from rapidly hyrolysed organic sources extracted from ³²P-labelled bacterial cultures. Plant Soil. 190(1):p. 163-167.
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