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| Web URL(s): | https://academic.oup.com/jee/article/90/6/1521/2216770/Carboxylamidase-Activity-in-the-Fall-Armyworm
Last checked: 02/28/2017
Access conditions: Item is within a limited-access website
Notes: Guide page |
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| Publication Type:
| Refereed |
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| Author(s): | Yu, Simon J.;
Valles, Steven M. |
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| Author Affiliation: | Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611 |
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| Title: | Carboxylamidase activity in the fall armyworm (Lepidoptera: Noctuidae) and other Lepidoptera, Orthoptera, and Dictyoptera |
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| Section: | Ecotoxicology
Other records with the "Ecotoxicology" Section
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| Source: | Journal of Economic Entomology. Vol. 90, No. 6, December 1997, p. 1521-1527. |
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| Publishing Information: | Lanham, MD: Entomological Society of America |
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| # of Pages: | 7 |
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| Keywords: | TIC Keywords: Spodoptera frugiperda; Ecology; Enzymes; Lepidoptera; Noctuidae; Orthoptera; Toxicology; Enzyme inhibitors
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| Abstract/Contents: | "Carboxylamidase activity in larvae of the fall armyworm, Spodoptera frugiperda (J. E. Smith), was studied using p-nitroacetanilide as a model substrate. Enzyme activity was found in all tissues examined, with the midgut exhibiting the highest specific activity. Of the midgut subcellular fractions, the microsomal fraction contained the highest specific activity. The apparent K^D[m value for the enzyme was 0.67 mM and the pH optimum was 8. The enzyme was quite stable, losing < 10% of its original activity after 10 wk at -20° C. Activity was inhibited in vitro by the hydrolase inhibitors paraoxon, triphenyl phosphate, eserine, and phenylmethylsulfonyl fluoride, showing (₅₀ values of 2.8 μM, 0.19 mM, 24 μM, and 0.13 mM, respectively. Activity was also inhibited by several organophosphorus insecticides at 0.1 mM; profenofos inhibited the enzyme 93%, dichlorvos 94%, and tetrachlorvinphos 75%. Carboxylamidase activity was expressed in all postembryonic stages. It was widely distributed in different insect species from the orders Lepidoptera, Orthoptera, and Dictyoptera. In the cockroach species studied, activity appeared to be family dependent. Activity was highest among the Blattelidae, intermediate along the Blaberidae, and not detectable in the Blattidae. Carboxylamidase activity toward other aromatic amides such as acetanilide and phenacetin was also detected in fall armyworm larvae." |
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| Language: | English |
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| References: | 27 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Yu, S. J., and S. M. Valles. 1997. Carboxylamidase activity in the fall armyworm (Lepidoptera: Noctuidae) and other Lepidoptera, Orthoptera, and Dictyoptera. J. Econ. Entomol. 90(6):p. 1521-1527. |
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| Web URL(s):
https://academic.oup.com/jee/article/90/6/1521/2216770/Carboxylamidase-Activity-in-the-Fall-Armyworm
Last checked: 02/28/2017
Access conditions: Item is within a limited-access website
Notes: Guide page |
| 
MSU catalog number: SB 931 .A1 J6 |
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