Full TGIF Record # 41955
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Web URL(s):http://apsjournals.apsnet.org/doi/pdfplus/10.1094/PHYTO.1998.88.2.129
    Last checked: 08/26/2010
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Publication Type:
i
Refereed
Author(s):Lo, C.-T.; Nelson, E. B.; Hayes, C. K.; Harman, G. E.
Author Affiliation:Lo, Hayes, and Harman, Departments of Plant Pathology and Horticultural Sciences, Cornell University, Geneva, NY 14456; Nelson, Department of Plant Pathology, Cornell University, Ithaca, NY 14853
Title:Ecological studies of transformed Trichoderma harzianum strain 1295-22 in the rhizosphere and on the phylloplane of creeping bentgrass
Section:Biological Control
Other records with the "Biological Control" Section
Source:Phytopathology. Vol. 88, No. 2, February 1998, p. 129-136.
Publishing Information:St. Paul, MN: American Phytopathological Society
# of Pages:8
Keywords:TIC Keywords: Trichoderma harzianum; Rhizosphere; Agrostis stolonifera; Rhizoctonia solani; Biological control
Abstract/Contents:"A ẞ-glucruronidase (GUS) reporter gene and a hygromycin B (hygB) phosphotransferase gene were integrated separately into the Trichoderma harzianum strain 1295-22 genome, using biolistic transformation. The mycelial growth and biocontrol ability of the transformed strains did not differ from that of the original strain. The transformed Gus+-kanamycin-resistant (Gus+Kan^D]R) strains were used to monitor growth and interactions with Rhizoctonia solani on creeping bentgrass plants. The hygB-resistant (hygB^D]R) strains were used to selectively recover strain 1295-22 from the rhizosphere soil and phylloplane of creeping bentgrass after spray applications. The population levels of two hygB^D]R strains and the original strain were very similar for all treatments. All three strains persisted for the duration of the experiment (28 days) in both the rhizoshpere soil and on leaves, although population levels declined somewhat over the course of the experiment in unautoclaved soils. In this study, the results demonstrated that hygB^D]R strains remained dominant over time when assayed on Trichoderma-selective medium containing hygB. The hygB^D]R strains were not displaced by strains that colonized untreated plants. Microscopic observation showed that the Gus+Kan^D]R strains colonized the rhizoplane, seed coat, and phylloplane of creeping bentgrass. These results supported our earlier observation that strain 1295-22 was rhizosphere and phylloplane competent. Interactions between T. harzianum and R. solani were readily observed in situ and changed over time. Two types of reactions were found in these experiments. In the first type, sections of hyphae of R. solani near the hyphae of T. harzianum appeared damaged, and the pathogen appeared necrotic when viewed with a microscope. The second type, observed less frequently than the first type, was typical of mycoparasitism. The findings in this study provide new insight into the interactions between R. solani and T. harzianum, providing a basis for future research.
Language:English
References:37
Note:Figures
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lo, C.-T., E. B. Nelson, C. K. Hayes, and G. E. Harman. 1998. Ecological studies of transformed Trichoderma harzianum strain 1295-22 in the rhizosphere and on the phylloplane of creeping bentgrass. Phytopathology. 88(2):p. 129-136.
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http://apsjournals.apsnet.org/doi/pdfplus/10.1094/PHYTO.1998.88.2.129
    Last checked: 08/26/2010
    Requires: PDF Reader
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