Full TGIF Record # 428
Item 1 of 1
Publication Type:
i
Refereed
Author(s):Torello, W. A.; Rufner, R.; Symington, A. G.
Author Affiliation:Torello: Assistant Professor; Rufner: Electron Microscopy Laboratory Director; Symington: Research Technician, Department of Plant and Soil Sciences, University of Massachusetts, Amherst, Massachusetts
Title:The ontogeny of somatic embryos from long-term callus cultures of red fescue
Section:Reports
Other records with the "Reports" Section
Source:HortScience. Vol. 20, No. 5, October 1985, p. 938-942.
Publishing Information:St. Joseph, MI : American Society for Horticultural Science
# of Pages:5
Keywords:TIC Keywords: Ontogeny; Calli; Festuca rubra subsp. rubra; 2,4-D; Embryogenesis; Regeneration; Tissue culture
Abstract/Contents:"Callus cultures initiated from mature caryopses of 'Dawson' red fescue (Festuca rubra L. var trichophylla Gaud.) and maintained on a Murashige and Skoog (MS) medium supplemented with 20μM of 2,4-D for 18 months retained a high capacity for plant regeneration through somatic embryogenesis. Embryogenic (E) callus tissue was white and compact and usually developed in sectors between friable and translucent nonembryogenic (NE) callus tissue. Total E callus production was 64% after 12 weeks of culture, 32% after 20 weeks, which then stabilized between 18% and 26% for the next 50 weeks. Scanning electron microscopy of E calli after 6, 12, and 18 month culture periods revealed normal and aberrant somatic embryos at all stages of development. After 18 months of culture, all regenerated plants appeared typical of red fescue with the exception of 8% to 12% regeneration of albino plants."
Language:English
References:20
See Also:Other items relating to: 2, 4 - D in Turf
Note:Pictures, b/w
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Torello, W. A., R. Rufner, and A. G. Symington. 1985. The ontogeny of somatic embryos from long-term callus cultures of red fescue. HortScience. 20(5):p. 938-942.
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