Full TGIF Record # 42840
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Web URL(s):http://www.tandfonline.com/doi/abs/10.1300/J099v02n03_05#.UqnNjeKQO8E
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Publication Type:
i
Report
Author(s):Inokuma, Chie; Sugiura, Kiyoyuki; Okawara, Ryoji; Cho, Chol
Author Affiliation:Japan Turfgrass Inc., Japan
Title:Transient gene expression in Japanese lawngrass (Zoysia japonica Steud.) protoplasts
Source:Journal of Turfgrass Management. Vol. 2, No. 3, 1997, p. 51-60.
# of Pages:10
Publishing Information:Binghamton, NY: Food Products Press (Haworth Press)
Keywords:TIC Keywords: Polyethylene glycol; Protoplasts; Transgene expression; Zoysia japonica
Abstract/Contents:"Conditions for maximizing transient expression and the activity of four promoter/intron constructs in protoplasts of Japanese lawngrass (Zoysia japonica Steud.) were investigated. It was found from the transient expression of the GUS reporter gene that polyethylene glycol (PEG)-mediated plasmid uptake largely depended on PEG concentration, plasmid quantity, and heat shock treatment of protoplasts before the addition of PEG. In the transient assay with four constructs, rice actin 1 promoter showed the highest level of transgene expression, suggesting that it is efficient for use in Japanese lawngrass transformation."
Language:English
References:17
Note:Figures
Tables
Graphs
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Inokuma, C., K. Sugiura, R. Okawara, and C. Cho. 1997. Transient gene expression in Japanese lawngrass (Zoysia japonica Steud.) protoplasts. J. Turfgrass Manage. 2(3):p. 51-60.
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Web URL(s):
http://www.tandfonline.com/doi/abs/10.1300/J099v02n03_05#.UqnNjeKQO8E
    Last checked: Item not verified
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
    Notes: Abstract and Guide page only
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MSU catalog number: SB 433 .J68
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