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| Web URL(s): | http://www.tandfonline.com/doi/abs/10.1300/J099v02n03_05#.UqnNjeKQO8E
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| Publication Type:
| Report |
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| Author(s): | Inokuma, Chie;
Sugiura, Kiyoyuki;
Okawara, Ryoji;
Cho, Chol |
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| Author Affiliation: | Japan Turfgrass Inc., Japan |
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| Title: | Transient gene expression in Japanese lawngrass (Zoysia japonica Steud.) protoplasts |
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| Source: | Journal of Turfgrass Management. Vol. 2, No. 3, 1997, p. 51-60. |
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| Publishing Information: | Binghamton, NY: Food Products Press (Haworth Press) |
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| # of Pages: | 10 |
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| Keywords: | TIC Keywords: Polyethylene glycol; Protoplasts; Transgene expression; Zoysia japonica
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| Abstract/Contents: | "Conditions for maximizing transient expression and the activity of four promoter/intron constructs in protoplasts of Japanese lawngrass (Zoysia japonica Steud.) were investigated. It was found from the transient expression of the GUS reporter gene that polyethylene glycol (PEG)-mediated plasmid uptake largely depended on PEG concentration, plasmid quantity, and heat shock treatment of protoplasts before the addition of PEG. In the transient assay with four constructs, rice actin 1 promoter showed the highest level of transgene expression, suggesting that it is efficient for use in Japanese lawngrass transformation." |
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| Language: | English |
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| References: | 17 |
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| Note: | Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Inokuma, C., K. Sugiura, R. Okawara, and C. Cho. 1997. Transient gene expression in Japanese lawngrass (Zoysia japonica Steud.) protoplasts. J. Turfgrass Manage. 2(3):p. 51-60. |
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| Web URL(s):
http://www.tandfonline.com/doi/abs/10.1300/J099v02n03_05#.UqnNjeKQO8E
Last checked: Item not verified
Requires: PDF Reader
Access conditions: Item is within a limited-access website
Notes: Abstract and Guide page only |
| 
MSU catalog number: SB 433 .J68 |
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