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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Baker, R. D.;
McCarty, L. B.;
Shilling, D. G.;
Colvin, D. L. |
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| Author Affiliation: | University of Florida, Gainesville, FL |
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| Title: | Differential susceptibility of bahiagrass (Paspalum notatum Flugge) varieties to metsulfuron methyl |
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| Section: | Paper and Abstracts Presented
Other records with the "Paper and Abstracts Presented" Section
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| Meeting Info.: | 49th Annual Meeting, Charlotte, NC, January 15-17, 1996 |
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| Source: | Southern Weed Science Society Proceedings. Vol. 49, 1996, p. 61. |
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| Publishing Information: | Champaign, IL: Southern Weed Science Society. |
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| # of Pages: | 1 |
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| Abstract/Contents: | "Field and greenhouse trials have shown certain varieties of bahiagrass (Paspalum notatum Flugge) to be tolerant to metsulfuron methyl. Argentine, Common, and Paraguayan varieties were not susceptible to the recommended rate of 13.9 g ha⁻1 which eliminated Pensacola and Tifton-9 varieties. Greenhouse studies were performed to determine the basis of the tolerance. The age of bahiagrass at the time of application had no effect on the tolerance. Varying the site of herbicide uptake resulted in only Pensacola and Tifton-9 varieties being susceptible to the recommended rate of metsulfuron methyl. Mixed-function oxidases are responsible for metsulfuron methyl detoxification in tolerant crops and are deactivated by organophosphate insecticides. An organophosphate insecticide was applied in conjunction with metsulfuron methyl to determine the role of mixed-function oxidases in the bahiagrass varietal tolerance. No change in tolerance level was shown. Argentine, Common, and Paraguayan were consistently tolerant to the recommended rate. Tolerant varieties were discovered to be tetraploid (2n=40) with susceptible varieties diploid (2n=20). Tolerant varieties could have ability to produce greater amounts of the target enzyme, acetolactate synthase (ALS). Laboratory studies were initiated to determine the amount of ALS activity present in each variety. Also, the effect of various concentrations of metsulfuron methyl on enzyme activity was investigated. Proteins were extracted from bahiagrass shoots (2-4 cm tall), and the product of the ALS reaction (acetolactate) was measured according to the Westerfield method for detection of acetoin. Reaction product was measured without inhibitor and in the presence of various concentrations of metsulfuron methyl: 1, 10, and 100 nM. Total protein extracted was determined using the Bradford method. Studies were randomized complete block design with three replications. Means separation performed according to Fisher's LSD (P=0.05). Argentine, Common, and Paraguayan varieties showed greater production of acetolactate than did Pensacola. Also, activity of ALS from Pensacola decreased more in the presence of 100 nM metsulfuron methyl compared to Common and Paraguayan varieties. Trends similar to those shown in the field were exhibited at the enzyme level. Future work will give further insight into the bahiagrass varietal tolerance." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Baker, R. D., L. B. McCarty, D. G. Shilling, and D. L. Colvin. 1996. Differential susceptibility of bahiagrass (Paspalum notatum Flugge) varieties to metsulfuron methyl. South. Weed Sci. Soc. Proc. 49:p. 61. |
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MSU catalog number: SB 611 .S6 |
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