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| Publication Type:
| Report |
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| Author(s): | Zeng, I.;
Baird, W. V. |
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| Author Affiliation: | Horticulture Department, Clemson University, Clemson, SC 29634 |
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| Title: | Identification of a molecular marker linked to dinitroaniline herbicide resistance in goosegrass |
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| Section: | Papers and Abstracts Presented
Other records with the "Papers and Abstracts Presented" Section
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| Meeting Info.: | 49th Annual Meeting, Charlotte, NC, January 15-17, 1996 |
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| Source: | Southern Weed Science Society Proceedings. Vol. 49, 1996, p. 141-145. |
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| Publishing Information: | Champaign, IL: Southern Weed Science Society. |
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| # of Pages: | 5 |
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| Abstract/Contents: | "Dinitroaniline herbicides (DNHs) disrupt microtubule arrays and are commonly used to control weeds in cotton, soybean and turf. DNH-resistance has been reported in both annual and perennial weed species, and, throughout the southeastern United States, commonly in goosegrass [Eleusine indica (L.) Gaertn.] populations. A molecular marker, diagnositc for the DNH-response phenotype(s), can add an option to the currently utilized bioassay for the identification of DNH-resistant mutants in natural and agricultural populations of goosegrass. Crosses were made between a resistant (R) biotype and a susceptible (S) biotype. F1 hybrids were identified by their heterozygous isoenzyme patterns. An F2 population was developed and the genotype of the F2 plants was inferred from the DNH-response phenotype (R or S) of their F3 progeny seedlings. The random amplified polymorphic DNA (RAPD) assay in conjunction with bulked segregant analysis was used to detect nucleotide sequence polymorphisms specific for DNH-phenotype(s) in goosegrass, and screen for RAPD markers linked to the DNH-response locus. Over three hundred oligonucleotide primers were screened, and two were found to amplify a polymorphic band between the two DNH-response (S and R) bulks. The two RAPD markers appeared to be dominant and linked in coupling to the DNH-response locus. One marker appears to be diagnostic for the DNH-response phenotypes in the population from which the parental lines were developed. We are cloning this RAPD fragment for use in DNA sequencing, and to develop a sequence-characterized amplified region (SCAR) marker in order to facilitate its specific use in characterizing other goosegrass populations from a wider geographic range." |
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| Language: | English |
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| References: | 12 |
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| Note: | Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zeng, I., and W. V. Baird. 1996. Identification of a molecular marker linked to dinitroaniline herbicide resistance in goosegrass. South. Weed Sci. Soc. Proc. 49:p. 141-145. |
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