Full TGIF Record # 43115
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Web URL(s):http://www.tandfonline.com/doi/abs/10.1080/01904169409364749
    Last checked: 10/13/2015
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Publication Type:
i
Report
Author(s):Kuo, Yu-Jen; Smith, M. A. L.; Spomer, L. Art
Author Affiliation:Graduate Assistant, Associate Professor and Professor, Department of horticulture, University of Illinois
Title:Merging callus level and whole plant microculture to select salt-tolerant `Seaside' creeping bentgrass
Source:Journal of Plant Nutrition. Vol. 17, No. 4, March 1994, p. 549-560.
# of Pages:12
Publishing Information:New York: Marcel Dekker, Inc.
Keywords:TIC Keywords: Agrostis stolonifera; Salt tolerance; Calli; Whole plant microculture; Tissue culture; Breeding
Cultivar Names:Seaside
Abstract/Contents:"More efficient methods for developing and identifying new salt-toleraant (ST) turfgrasses are needed since salt from irrigation is accumulating in production and recreation soils. In this study, callus culture selection was combined with whole plant microculture (WPMC) screening to facilitate identifying ST 'Seaside' creeping bentgrass genotypes. Putative ST cell lines were first isolated from salt-challenged compact callus cultures grown in media containing 0-3% sodium sulfate (Na₂SO₄). These selected cell cultures were then regenerated and rescreened at the WPMC level in vitro to compare their vegetative growth relative to non-selected plants. Neither the selected nor the non-selected microcultured plants produced roots in an in vitro medium containing 3% Na₂SO₄. However, the selected plantlets exhibited slightly better root and shoot growth than non-selected plants at lower salinity (1.5-2.0% Na₂SO₄). This technique proved successful for screening potentially salt-tolerant turfgrasses. In addition to speeding selection, it may also prove useful in studies of whole plant ST mechanisms."
Language:English
References:24
Note:Pictures, b/w
Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Kuo, Y.-J., M. A. L. Smith, and L. A. Spomer. 1994. Merging callus level and whole plant microculture to select salt-tolerant `Seaside' creeping bentgrass. J. Plant Nutr. 17(4):p. 549-560.
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Web URL(s):
http://www.tandfonline.com/doi/abs/10.1080/01904169409364749
    Last checked: 10/13/2015
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
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