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| Web URL(s): | https://www.crops.org/publications/cs/pdfs/38/2/CS0380020478
Last checked: 08/05/2010
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| Publication Type:
| Refereed |
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| Author(s): | Johnson, P. G.;
Riordan, T. P.;
Arumuganathan, K. |
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| Author Affiliation: | Johnson and Riordan: Department of Horticulture, University of Nebraska, Lincoln, NE 68583-0724. Arumuganathan: Center for Biotechnology, University of Nebraska, Lincoln, NE 68583-0724. |
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| Title: | Ploidy level determinations in buffalograss clones and populations |
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| Section: | Cell biology and molecular genetics
Other records with the "Cell biology and molecular genetics" Section
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| Source: | Crop Science. Vol. 38, No. 2, March/April 1998, p. 478-482. |
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| Publishing Information: | Madison, WI: Crop Science Society of America |
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| # of Pages: | 5 |
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| Keywords: | TIC Keywords: Bouteloua dactyloides; Ploidy; Chromosome number; Identification
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| Abstract/Contents: | "Buffalograss [Buchloë diactyloides (Nutt,) Englem.] is the subject of breeding programs for turfgrass use because of its drought and heat tolerance, low growing habit, and low management input. Three ploidy levels are known to exist (x=n=10), but plants at each level are nearly morphologically indistinguishable. In this paper, flow cytometry was evaluated as a means of determining ploidy levels of 31 buffalograss clones and 34 seeded populations. Six clones were analyzed for nuclear DNA base-pair composition by flow cytometry. Based on DNA contents, buffalograss clones can be grouped into four sets corresponding to chromosome number or ploidy level. Mean DNA contents were 0.93, 1.80, 2.15, and 2.63 pg DNA/nucleus for diploid, tetraploid, pentaploid, and hexaploids, respectively. Chromosome numbers explained most of the DNA content variation as shown by the strong linear relationship between DNA content and chromosome number. Cultivar 315 is a pentaploid and is the first report of a pentaploid among buffalograss. Seeded populations analyzed include those exclusively tetraploid, mixture of tetraploid through hexaploid, and those exclusively hexaploid. Aneuploids between pentaploid and hexaploid levels were noted. No differences were observed between ploidy levels or genders with regard to base-pair composition." |
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| Language: | English |
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| References: | 27 |
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| Note: | Pictures, b/w
Tables
Figures |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Johnson, P. G., T. P. Riordan, and K. Arumuganathan. 1998. Ploidy level determinations in buffalograss clones and populations. Crop Sci. 38(2):p. 478-482. |
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| Web URL(s):
https://www.crops.org/publications/cs/pdfs/38/2/CS0380020478
Last checked: 08/05/2010
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: SB 183 .C7 |
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