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| Web URL(s): | http://www.newss.org/proceedings/proceedings_1998_vol52.pdf#page=35
Last checked: 07/24/2013
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Mitra, S.;
Bhowmik, P. C.;
Bernatzky, R. |
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| Author Affiliation: | Graduate Research Assistant, Professor of Weed Science and Associate Professor, Department of Plant and Soil Sciences, University of Massachusetts, Amherst, MA 01003 |
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| Title: | DNA profiles of different biotypes of quackgrass (Elytrigia repens) |
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| Section: | Agronomy Tuesday-January 6, 1998
Other records with the "Agronomy Tuesday-January 6, 1998" Section
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| Meeting Info.: | Washington, DC: January 5-8, 1998 |
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| Source: | Proceedings of the 52nd Annual Meeting of the Northeastern Weed Science Society. Vol. 52, 1998, p. 35. |
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| Publishing Information: | College Park, MD: Northeastern Weed Science Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Biotypes; Comparisons; Cultivar profile; DNA extraction; Elymus repens; Susceptibility
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| Abstract/Contents: | "Ecotypes or biotypes of quackgrass have been found to exist in North America and different parts of the world. Biotypes of quackgrass have been reported to have different susceptibility to various herbicides. The difference in susceptibility can be explained by their phenotypic characteristics like size of leaf blade, length of leaf blade or their molecular makeup. In order to study the molecular structures DNA extraction and analysis has become popular. Two biotypes of quackgrass were collected from Amherst, MA and Lebanon, NJ. DNA was extracted from 0.1 gm of leaves and rhizomes with a Phytopure plant DNA extraction kit (Nucleon® biosciences, Lanarkshire, UK). The objective was to examine DNA from different plant organs (leaves vs rhizomes) of the same plant and to study the yield from these two sources. Nucleon® Phytopure resin which contains free boric acid was used in the DNA extraction protocol. The RNA in the samples were removed by the addition of 1 μl of RNAse followed by an incubation at 37 C. DNA was checked by electrophoresis on a agarose gel and the yields were estimated comparing them to 50 and 100 ng of standard DNA extract. Absorbance of the DNA extract at 260 nm wavelength was recorded. DNA extracts were then amplified using polymeraze chain reaction (PCR) at an annealing temperature cycle of 94, 37, and 72 C for 40 cycles. These primers, OP A-7, A-10 and A-13 (Operon Technology, CA) were used with samples at two concentrations (1 and 0.1ng/2μl) of DNA extracts. Based on the absorbance at 260 nm, the yields of DNA from the quackgrass leaves was 50% more than the rhizomes. At the particular annealing temperature cycles primer A-10 did not give us any difference between the banding pattern of the two biotopes." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Mitra, S., P. C. Bhowmik, and R. Bernatzky. 1998. DNA profiles of different biotypes of quackgrass (Elytrigia repens). Proc. Annu. Meet. Northeast. Weed Sci. Soc. 52:p. 35. |
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| Web URL(s):
http://www.newss.org/proceedings/proceedings_1998_vol52.pdf#page=35
Last checked: 07/24/2013
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: SB 610 .N62 v. 52 |
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