| |
| DOI: | 10.1104/pp.72.3.754 |
|---|
| Web URL(s): | http://www.plantphysiol.org/content/72/3/754.full.pdf+html
Last checked: 08/09/12
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
|---|
| Publication Type:
| Refereed |
|---|
| Author(s): | Cohen, Peter B.;
Schibeci, Angelo;
Fincher, Geoffrey B. |
|---|
| Author Affiliation: | Department of Biochemistry, La Trobe University, Bundoora, Victoria, Australia |
|---|
| Title: | Subcellular distribution of prolyl hydroxylase |
|---|
| Article Series: | Biosynthesis of arabinogalactan-protein in Lolium multiflorum (ryegrass) endosperm cells, part 3 |
|---|
| Source: | Plant Physiology. Vol. 72, No. 3, July 1983, p. 754-758. |
|---|
| # of Pages: | 5 |
|---|
| Related Web URL: | http://www.plantphysiol.org/content/72/3/754.abstract
Last checked: 08/09/12
Notes: Abstract only |
|---|
| Keywords: | TIC Keywords: Lolium multiflorum; Enzymes; Proteins; Metabolism; Proline; Fractionation; Endoplasmic reticulum; Hydroxylases
|
|---|
| Abstract/Contents: | "The peptidyl prolyl hydroxylase responsible for the formation of hydroxyproline during arabinogalactan-protein biosynthesis in Lolium multiflorum (ryegrass) endosperm cells is a membrane-associated enzyme which will catalyze the hydroxylation of poly(L-proline) in the presence of oxygen, a-ketoglutarate, ferrous ion, and ascorbate. The Km for poly(L-proline) (8000 molecular weight) is 40 micromolar. The enzyme will also hydroxylate the protocollagen analog (Pro-Pro-Gly)5·4H2O. Fractionation of membranes from protoplast lysates on a discontinuous sucrose/sorbitol density gradient, followed by centrifugation on a linear sucrose gradient in the presence of Mg2+, leads to a clear separation of a number of membrane components. The membrane components have been tentatively identified using marker enzymes and assayed for peptidyl prolyl hydroxylase. It is concluded that the ryegrass prolyl hydroxylase is enriched in Golgi-derived membranes, but that significant amounts are also located in other subcellular fractions, including the rough endoplasmic reticulum." |
|---|
| Language: | English |
|---|
| References: | 30 |
|---|
| See Also: | See also part 1 "Hydroxylation of peptidyl proline" Australian Journal of Plant Physiology 8(2) 1981, p. 121-132, R=5100. R=5100
See also part 2 "In vitro incorporation of galactosyl residues from UDPgalactose into polymeric products" Australian Journal of Plant Physiology 9(1) 1982, p. 31-45, R=5074. R=5074
See also part 4 "Subcellular distribution of galactosyltransferases" Biochemical Journal 218(2) 1984, p. 633-636, R=4297. R=4297 |
|---|
| Note: | Tables
Graphs |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Cohen, P. B., A. Schibeci, and G. B. Fincher. 1983. Subcellular distribution of prolyl hydroxylase. Plant Physiol. 72(3):p. 754-758. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=5049 |
| If there are problems with this record, send us feedback about record 5049. |
| Choices for finding the above item: |
|---|
| DOI: 10.1104/pp.72.3.754 |
| Web URL(s):
http://www.plantphysiol.org/content/72/3/754.full.pdf+html
Last checked: 08/09/12
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: QK 1 .P68 |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
