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Author(s): | Anderson, R. L.;
Stone, B. A. |
Author Affiliation: | Dep. of Biochem., La Trobe Univ., Bundoora, Vic., Australia 3083 |
Title: | Studies on Lolium multiflorum endosperm in tissue culture. 3. Structural studies on the cell walls |
Source: | Australian Journal of Biological Sciences. Vol. 31, No. 6, 1978, p. 573-586. |
Keywords: | TIC Keywords: Lolium multiflorum; Chemical composition
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Geographic Terms: | Australia, Victoria |
Abstract/Contents: | Cell wall preparations from Italian ryegrass endosperm cells grown in tissue culture were analysed for residual glucans and other polysaccharides. Stationary phase cells contained 3.0% protein, 14% 1,3-glucose and 49% 1,4-glucose residues: xylose, arabinose and galactose were also present. The amino acid composition of the protein is given. The cell wall preparations were fractionated by successive extraction with water at 40 deg C, 8 M urea, 0.5 M KOH, 4.27 M KOH and 6 M NaOH/0.81 M H3BO3. The 3 main polysaccharides identified in the fractions were 1,3:1,4- beta -glucan, arabinoxylan and/or galacto-arabinoxylan and cellulose. The mixed-linked glucans and arabinoxylans were present in the 8 M urea and 0.5 and 4.27 M KOH fractions. The presence of hydroxylamine-sensitive linkages in the wall was confirmed. |
Language: | English |
References: | 47 |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Anderson, R. L., and B. A. Stone. 1978. Studies on Lolium multiflorum endosperm in tissue culture. 3. Structural studies on the cell walls. Aust. J. Biol. Sci. 31(6):p. 573-586. |
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