Full TGIF Record # 52580
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Web URL(s):https://www.crops.org/publications/cs/pdfs/38/3/CS0380030801
    Last checked: 08/05/2010
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Publication Type:
i
Refereed
Author(s):Warnke, S. E.; Douches, D. S.; Branham, B. E.
Author Affiliation:NFSPRC, 3450 S.W. Campus Way, Corvallis, OR 97331; Department of Crop and Soil Science, Michigan State University, East Lansing, MI 48823; and Department of Natural Resources and Environmental Sciences, 1102 S. Goodwin Dr., Urbana, IL 61801
Title:Isozyme analysis supports allotetraploid inheritance in tetraploid creeping bentgrass (Agrostis palustris Huds.)
Section:Turfgrass science
Other records with the "Turfgrass science" Section
Source:Crop Science. Vol. 38, No. 3, May/June 1998, p. 801-805.
Publishing Information:Madison, WI: Crop Science Society of America
# of Pages:5
Keywords:TIC Keywords: Agrostis stolonifera; Inheritance; Ploidy
Abstract/Contents:"Inheritance data from tetraploid Agrostis palustris Huds. populations produced by crossing selected plants from cultivated creeping bentgrass varieties is reported. Phosphoglucose isomerase, phosphoglucomutase, glutamate oxaloacetate transaminase, triosphosphate isomerase, anodal peroxidase, esterase, and alcohol dehydrogenase were resolved in a Tris-citrate/lithium-borate pH 8.3 buffer system. Acid phosphatase, 6-phosphogluconate dehydrogenase, aconitase, malate dehydrogenase (MDH), and shikimic acid dehydrogenase were resolved in a histidine-citrate pH 5.7 buffer system. Four enzyme systems exhibiting polymorphism in the Tris-citrate/lithium-borate buffer system were selected for further genetics studies. Segregation data at the Tpi-1, Tpi-2, Got-2, Pgi-2, and Pgm-s loci were used to identify allozymes and distinguish between allo- or autotetraploid segregation patterns. Two allozymes were identified at the Pgm-2 and Tpi-1 loci, while three allozymes were identified at the Got-2 and Tpi-2 loci. The Pgi-2 locus was highly polymorphic with six different allozymes identified. Fixed heterozygosity at the Tpi-2 and Got-2 loci and disomic segregation for a tetra-allelic individual at the Pgi-2 locus provided strong genetic evidence to support allotetraploid inheritance in creeping bentgrass. Additionally, one parental clone that exhibited high levels of self-fertility was identified and its breeding implications are discussed."
Language:English
References:24
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Warnke, S. E., D. S. Douches, and B. E. Branham. 1998. Isozyme analysis supports allotetraploid inheritance in tetraploid creeping bentgrass (Agrostis palustris Huds.). Crop Sci. 38(3):p. 801-805.
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Web URL(s):
https://www.crops.org/publications/cs/pdfs/38/3/CS0380030801
    Last checked: 08/05/2010
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
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