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| Publication Type:
| Report |
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| Author(s): | Yuen, G. Y.;
Zhang, Z.;
Giesler, L. J. |
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| Author Affiliation: | Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583 |
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| Title: | Bipolaris leaf spot biocontrol with a bacterial agent, 1997 |
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| Section: | Turfgrasses
Other records with the "Turfgrasses" Section
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| Source: | Biological and Cultural Tests for Control of Plant. Vol. 13, 1998, p. 142. |
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| Publishing Information: | St. Paul, MN: The American Phytopathological Society |
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| # of Pages: | 1 |
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| Abstract/Contents: | "Stenotrophomonas maltophilia strain C3, a chitinolytic strain isolated from Kentucky bluegrass foliage, was evaluated in two experiments. the experiments were conducted in established tall fescue turf at the University of Nebraska John Seaton Anderson Turfgrass Research Facility near Mead, NE. The first experiment was located in 'Kentucky 31', the second in 'Wrangler'. Both plantings were maintained under low fertility (around 50 kg N/ha annually), twice-weekly irrigation, and a 8 cm height. Urea (50 kg/ha) was applied to the experiment areas about 2 wk before each experiment. There were four 1.5 m x 1.5 m replicate plots per treatment arranged in a randomized complete block design. Nontreated plots were the controls. In the first experiment, 250 ml of a C3 cell suspension (7 X 10E8 CFU/ml in phosphate buffer amended with 0.25% Soydex) was applied to plots on 2 Jun and a second application was made 18 days later. All plots were inoculated on 9 Jun with 250 ml of a B. sorokiniana conidial suspension containing 5 X 10E5 spores/ml in the buffer solution. The plots were sprinkler-irrigated shortly after inoculation. Because there were drying winds and humidity was low, the experiment area was covered with Seed Guard tarp for 2 days to maintain high canopy moisture. In the second experiment, C3 was tested at 10E7 CFU/ml, with 500 ml of suspension being applied to each plot. In addition, C3 at 10E7 CFU/ml in combination with 0.4% (w/v) colloidal chitin was tested. The treatments were applied on 2 Sep and 9 Sep. Pathogen inoculum (500 ml/plot) was sprayed on 5 Sep. The experiment was irrigated just prior to inoculation, and ambient humidity was sufficient for infection. Foliage was sampled 11 and 7 days after pathogen inoculation in experiment 1 and 2, respectively, and again 1 wk later. Three subsamples of over 10 leaves each were collected from each plot and then pooled. Total numbers of lesions and total leaf length were measured in each sample and used in calculating infection frequency (numbers of lesions per 100 cm leaf). In addition, the percent diseased leaf area in each sample was estimated. Infection frequencies in both experiments were high. Lesions did not expand appreciably in size, however, and so the percent diseased leaf area was low at the end of both experiments. When disease severity (percent diseased turf area) was gauged across entire plots, treatment effects could not be discrened. All treatments with strain C3 reduced infection frequency and diseased leaf area compared to the controls. In the second experiment, the highest degree of control was provided by the high cell density treatment. The addition of chitin enhanced the efficacy of the low cell density treatment." |
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| Language: | English |
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| References: | 0 |
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| Note: | Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Yuen, G. Y., Z. Zhang, and L. J. Giesler. 1998. Bipolaris leaf spot biocontrol with a bacterial agent, 1997. Biol. Cult. Tests Control Plant Dis. 13:p. 142. |
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