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| Web URL(s): | http://ac.els-cdn.com/S0022201185711032/1-s2.0-S0022201185711032-main.pdf?_tid=e1b5675c-6c51-11e5-b62b-00000aacb362&acdnat=1444153644_f6f05ae3887f43261254dc8469e7c585
Last checked: 10/06/2015
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| Publication Type:
| Refereed |
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| Author(s): | Hashmi, Sarwar;
Hashmi, Ghazala;
Graughler, Randy |
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| Author Affiliation: | Department of Entomology, Rutgers University, New Brunswick, New Jersey 08903 |
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| Title: | Genetic transformation of an entomopathogenic nematode by microinjection |
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| Source: | Journal of Invertebrate Pathology. Vol. 66, No. 3, November 1995, p. 293-296. |
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| Publishing Information: | San Diego: Academic Press |
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| # of Pages: | 4 |
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| Keywords: | TIC Keywords: Genetic transformation; Entomopathogenic nematodes; Heterorhabditis bacteriophora
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| Abstract/Contents: | "We report the first successful transformation of an entomopathogenic nematode. Foreign genes were introduced in Heterorhabditis bacteriophora HP88 by microinjection using vectors carrying the Caenorhabditis elegans genes coding for the roller phenotype and 16-kDa heat shock protein (hsp16) gene. A translational fusion made by inserting lacZ in frame into hsp16 was expressed in the body masculature, hypodermis, and pharyngeal muscles. The transcription of the hsp16/lacZ transgenes resulted in the rapid synthesis of detectable levels of Β-glalctosidase. This research opens new avenues for genetic modification of entomopathogenic nematodes." |
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| Language: | English |
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| References: | 22 |
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| Note: | Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Hashmi, S., G. Hashmi, and R. Graughler. 1995. Genetic transformation of an entomopathogenic nematode by microinjection. J. Invertebr. Pathol. 66(3):p. 293-296. |
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| Web URL(s):
http://ac.els-cdn.com/S0022201185711032/1-s2.0-S0022201185711032-main.pdf?_tid=e1b5675c-6c51-11e5-b62b-00000aacb362&acdnat=1444153644_f6f05ae3887f43261254dc8469e7c585
Last checked: 10/06/2015
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Access conditions: Item is within a limited-access website |
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MSU catalog number: SB 942 .J6 |
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