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| Web URL(s): | https://archive.lib.msu.edu/tic/its/articles/1997jou291.pdf
Last checked: 09/29/2008
Requires: PDF Reader |
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| Access Restriction: | Certain MSU-hosted archive URLs may be restricted to legacy database members. |
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| Publication Type:
| Refereed |
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| Author(s): | Golembiewski, R. C.;
Danneberger, T. K.;
Sweeney, P. M. |
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| Author Affiliation: | Department of Horticulture and Crop Science, The Ohio State University, Columbus, Ohio USA |
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| Title: | RAPD analysis of creeping bent seed and leaf tissue |
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| Section: | Breeding and genetics: Contributed papers
Other records with the "Breeding and genetics: Contributed papers" Section
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| Meeting Info.: | Sydney, Australia: 1997 |
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| Source: | International Turfgrass Society Research Journal. Vol. 8, No. Part 1, 1997, p. 291-296. |
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| Publishing Information: | Blacksburg, VA: International Turfgrass Society |
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| # of Pages: | 6 |
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| Keywords: | TIC Keywords: Random amplified polymorphic DNA markers; Agrostis stolonifera; Cultivar identification; Seeds; Leaf tissue
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| Abstract/Contents: | "As the use of random amplified polymorphic DNA (RAPD) markers for genotypic and cultivar identification increases, it will be important to relate field populations of creeping bent (Agrostis stolonifera var. stolonifera) to the seed populations used to establish the turfgrass. Therefore, it is important to determine whether RAPD markers that distinguish seed populations are reproducible in leaf tissue. RAPD markers that distinguish seed populations are reproducible in leaf tissue. RAPD markers that distinguish bulk seed samples of SR1020, Lopez, and Providence from 12 other creeping bent cultivars were used for the analysis. DNA was extracted from either a 200 mg bulk seed sample or seven day old leaf tissue (0.5 to 1 cm of each individual) resulting from the germination of 200 mg seed. Each cultivar showed the same banding patterns regardless of the tissue used for extraction. Notably, the RAPD markers unique to SR1020, Lopez, and Providence were observed in amplification products from both leaf and seed tissue of each particular cultivar, but were not present in either tissue of the other cultivars. Replicate extractions and amplifications produced identical results. This study illustrates the potential of using RAPD markers to compare seed and field populations of creeping bent." |
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| Language: | English |
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| References: | 14 |
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| Note: | Pictures, b/w
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Golembiewski, R. C., T. K. Danneberger, and P. M. Sweeney. 1997. RAPD analysis of creeping bent seed and leaf tissue. Int. Turfgrass Soc. Res. J. 8(Part 1):p. 291-296. |
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| Web URL(s):
https://archive.lib.msu.edu/tic/its/articles/1997jou291.pdf
Last checked: 09/29/2008
Requires: PDF Reader |
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MSU catalog number: SB 433 .I52 v. 8 |
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