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Web URL(s): | https://archive.lib.msu.edu/tic/its/articles/1997jou337.pdf Last checked: 08/04/2010 Requires: PDF Reader |
Access Restriction: | Certain MSU-hosted archive URLs may be restricted to legacy database members. |
Publication Type:
| Refereed |
Author(s): | Lee, L.;
Laramore, C.;
Hartman, C. L.;
Yang, L.;
Funk, C. R.;
Grande, J.;
Murphy, J. A.;
Johnston, S. A.;
Majek, B. A.;
Tumer, N. E.;
Day, P. R. |
Author Affiliation: | Lee: The Scotts Company, Marysville, Ohio USA; and Laramore, Yang, Tumer and Day: AgBiotech Center, Rutgers University, Cook College, New Brunswick, New Jersey USA; and Hartman: NJ AES, Office of IR-4 Headquarters, USA; and Funk: Dept. of Plant Science, Rutgers University, USA; and Grande: Synder Research and Extension Farm, Rutgers University, USA; and Johnston and Majek: Rutgers' Research and Development Center of Bridgeton, New Jersey USA |
Title: | Field evaluation of herbicide resistance in transgenic Agrostis stolonifera L. and inheritance in the progeny |
Section: | Breeding and genetics: Contributed papers Other records with the "Breeding and genetics: Contributed papers" Section
|
Meeting Info.: | Sydney, Australia: 1997 |
Source: | International Turfgrass Society Research Journal. Vol. 8, No. Part 1, 1997, p. 337-344. |
Publishing Information: | Blacksburg, VA: International Turfgrass Society |
# of Pages: | 8 |
Keywords: | TIC Keywords: Agrostis stolonifera; Genetic transformation; Herbicide resistance; Inheritance; Glufosinate
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Abstract/Contents: | "Herbicide resistant transgenic creeping bent lines were evaluated under field conditions in 1994 and 1995. These lines were obtained from biolistic and protoplast transformations with chimeric bar genes encoding phosphinothricin acetyltransferase. Vegetatively multiplied plants were planted in randomised plots. Herbicide treatments were at 0, 1x (0.84 kg ha⁻¹) and 3x (2.52 kg ha⁻¹) field rates of Glufosinate and replicated three times. All the transgenic plants that displayed complete herbicide resistance in greenhouse tests demonstrated a high level of herbicide resistance in field tests. They were unaffected by both 1x and 3x rates and looked green and healthy. Treated control plants were killed within 5-10 days after herbicide application. 37 plants, from biolistic transformation and 33 plants from protoplast transformation showed a high level of herbicide resistance. Seeds were harvested from vernalised transgenic plants pollinated with seed-grown control plants in a containment greenhouse. Transgenic seedlings were able to germinate in the presence of herbicide while control seeds of the same cultivar did not germinate in Petri dish germination tests. After transplanting to soil, transgenic seedlings were completely resistant to a 2 mg/ml Herbiace (equivalent to 3.36kg ha⁻¹ Bialaphos) treatment in a greenhouse test. Our results show that herbicide resistance was inherited in the seedlings." |
Language: | English |
References: | 6 |
Note: | Figures Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Lee, L., C. Laramore, C. L. Hartman, L. Yang, C. R. Funk, J. Grande, et al. 1997. Field evaluation of herbicide resistance in transgenic Agrostis stolonifera L. and inheritance in the progeny. Int. Turfgrass Soc. Res. J. 8(Part 1):p. 337-344. |
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| Web URL(s): https://archive.lib.msu.edu/tic/its/articles/1997jou337.pdf Last checked: 08/04/2010 Requires: PDF Reader |
| MSU catalog number: SB 433 .I52 v. 8 |
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