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| Publication Type:
| Refereed |
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| Author(s): | Chastagner, G. A.;
Hammer, W. |
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| Author Affiliation: | Chastagner: Plant pathologist, Department of Plant Pathology, Washington State University Research and Extension Center, Puyallup 98371, USA; and Hammer: The Land, EPCOT, Agricultural Office, P.O. Box 10,000, Lake Buena Vista, FL 32830, USA |
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| Title: | Factors affecting the in vitro production of pseudothecia of Leptosphaeria korrae |
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| Section: | Turf diseases: Contributed papers
Other records with the "Turf diseases: Contributed papers" Section
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| Meeting Info.: | Sydney, Australia: 1997 |
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| Source: | International Turfgrass Society Research Journal. Vol. 8, No. Part 1, 1997, p. 847-861. |
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| Publishing Information: | Blacksburg, VA: International Turfgrass Society |
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| # of Pages: | 15 |
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| Keywords: | TIC Keywords: Ophiosphaerella korrae; Development; In vitro; Growing media; Temperatures; Light; Osmotic potential
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| Abstract/Contents: | "The identification of ectotrophic root-infecting fungi associated with patch diseases of turfgrass is largely dependent upon examination of structures associated with the telemorph stage of these fungi. Pseudothecia of Leptosphaeria korrae, the casual agent of necrotic ring spot, are seldom seen in vivo. A series of replicated experiments were conducted to examine the effect of substrate ['Scaldis' hard fescue seedlings, colonised prosso millet seed, and colonised potato-dextrose agar mycelial plugs on 2% water agar (WA)]; temperature (10, 15, 20, 25Ā°C); light source (none, florescent, near ultra violet); light intensity (31 to 200 Ī¼E mā»Ā² sā»Ā¹); and osmotic potential (-0.06 to -8.0 MPa) on pseudothecial production by L. korrae in vitro. Fertile pseudothecia were produced at temperatures ranging from 15 to 25Ā°C with significantly more being formed at 20Ā°C. Pseudothecia were not produced in the dark and light intensity had very little effect on the number of pseudothecia produced. Pseudothecia were produced only at the highest osmotic potential tested, -0.06 MPa. A higher number of pseudothecia were produced on 'Scaldis' hard fescue seedlings than when millet seed or PDA mycelial plugs were placed on 2% WA. More pseudothecia were formed on the roots than the seed coat or leaf sheaths of the hard fescue seedlings. Approximately two-thirds of the 54 putative isolates of L. korrae from Washington, Michigan, New York, Utah, Colorado, and California that were tested formed pseudothecia on 'Scaldis' hard fescue seedlings. The capacity to produce pseudothecia, frequency with which isolates produced pseudothecia, and the average number of pseudothecia formed per plate varied depending on the site from which Washington isolates were obtained. Mean ascospore measurements ranged from 105-147 Ī¼m (62-211) by 4.8 Ī¼m (3.0-7.0) and spores had an average of 7 (2-14) septa." |
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| Language: | English |
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| References: | 35 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Chastagner, G. A., and W. Hammer. 1997. Factors affecting the in vitro production of pseudothecia of Leptosphaeria korrae. Int. Turfgrass Soc. Res. J. 8(Part 1):p. 847-861. |
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