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| Author(s): | Thomas, H. M.;
Morgan, W. G.;
Meredith, M. R.;
Humphreys, M. W.;
Thomas, H.;
Leggett, J. M. |
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| Author Affiliation: | AFRC Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB, UK |
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| Title: | Identification of parental and recombined chromosomes in hybrid derivatives of Lolium multiflorum X Festuca pratensis by genomic in situ hybridization |
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| Source: | Theoretical and Applied Genetics. Vol. 88, No. 8, September 1994, p. 909-913. |
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| Publishing Information: | Berlin: Springer |
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| # of Pages: | 5 |
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| Keywords: | TIC Keywords: Lolium multiflorum; Festuca pratensis; Hybridization; Genetic markers; Identification
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| Abstract/Contents: | "Genomic in situ hybridization (GISH) was used to identify Festuca chromatin in mitotoic chromosomes of Lolium multiflorum (Lm) X Festuca pratensis (Fp) hybrids and hybrid derivatives. In two inverse autoallotriploids LmLmFp and LmFpFp, in situ hybridization was able to discriminate between the Lolium and Festuca chromosomes. In a third triploid hybrid produced by crossing an amphiploid of L. multiflorum X F. pratensis (2n=4x=28) with L. multiflorum (2n=2x=14), the technique identified chromosomes with interspecific recombination. Also, in an introgressed line of L. multiflorum which was homozygous for the recessive sid (senescence induced degradation) allele from F. pratensis, a pair of chormosome segments carrying the sid gene could be identified, indicating the suitability of GISH in showing the presence and location of introgressed genes. By screening backcross progeny for the presence of critical alien segments and the absence of other segments the reconstitution of the genome of the recipient species can be accelerated." |
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| Language: | English |
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| References: | 16 |
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| Note: | Pictures, color |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Thomas, H. M., W. G. Morgan, M. R. Meredith, M. W. Humphreys, H. Thomas, and J. M. Leggett. 1994. Identification of parental and recombined chromosomes in hybrid derivatives of Lolium multiflorum X Festuca pratensis by genomic in situ hybridization. Theor. Appl. Genet. 88(8):p. 909-913. |
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