Full TGIF Record # 58449
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DOI:10.21273/HORTSCI.30.4.913G
Web URL(s):https://journals.ashs.org/hortsci/view/journals/hortsci/30/4/article-p913G.xml
    Last checked: 11/14/2019
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Publication Type:
i
Refereed
Author(s):Brown, Aaron; Hughes, Harrison G.
Author Affiliation:Department of Horticulture, Colorado State University, Fort Collins, CO 80523
Title:Transient Expression of Β-Glucuronidase (GUS) activity in embryonic callus tissue of alkaligrass (Puccinellia distans) using particle bombardment
Source:HortScience. Vol. 30, No. 4-7, July 1995, p. 913 (1224).
Publishing Information:Alexandria, VA: American Society for Horticultural Science
# of Pages:1
Keywords:TIC Keywords: Puccinellia distans; Calli; Regeneration; Genetic engineering; Techniques
Abstract/Contents:"Callus induction and regeneration of alkaligrass (Puccinellia distans) was developed in our laboratory for use in transformation studies of turfgrass. Particle bombardment of the embryonic callus is being evaluated using a helium particle inflow gun constructed at Colorado State University, according to the design of Philippe et al. (Ohio State University 1993). Its utility in delivering DNA to plant cells is being tested by measuring the frequency of transient gene expression of a reporter gene (GUS pBI121) in embryogenic callus of alkaligrass. Varying pressure of helium and the distance of the calli in the chamber are also being avaluated for efficiency in transformation."
Language:English
References:0
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Brown, A., and H. G. Hughes. 1995. Transient Expression of Β-Glucuronidase (GUS) activity in embryonic callus tissue of alkaligrass (Puccinellia distans) using particle bombardment. HortScience. 30(4-7):p. 913 (1224).
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DOI: 10.21273/HORTSCI.30.4.913G
Web URL(s):
https://journals.ashs.org/hortsci/view/journals/hortsci/30/4/article-p913G.xml
    Last checked: 11/14/2019
    Requires: PDF Reader
    Notes: Item is within a single large file
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MSU catalog number: SB 1 .H64
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