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DOI: | 10.21273/HORTSCI.30.4.913G |
Web URL(s): | https://journals.ashs.org/hortsci/view/journals/hortsci/30/4/article-p913G.xml Last checked: 11/14/2019 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Refereed |
Author(s): | Brown, Aaron;
Hughes, Harrison G. |
Author Affiliation: | Department of Horticulture, Colorado State University, Fort Collins, CO 80523 |
Title: | Transient Expression of Β-Glucuronidase (GUS) activity in embryonic callus tissue of alkaligrass (Puccinellia distans) using particle bombardment |
Source: | HortScience. Vol. 30, No. 4-7, July 1995, p. 913 (1224). |
Publishing Information: | Alexandria, VA: American Society for Horticultural Science |
# of Pages: | 1 |
Keywords: | TIC Keywords: Puccinellia distans; Calli; Regeneration; Genetic engineering; Techniques
|
Abstract/Contents: | "Callus induction and regeneration of alkaligrass (Puccinellia distans) was developed in our laboratory for use in transformation studies of turfgrass. Particle bombardment of the embryonic callus is being evaluated using a helium particle inflow gun constructed at Colorado State University, according to the design of Philippe et al. (Ohio State University 1993). Its utility in delivering DNA to plant cells is being tested by measuring the frequency of transient gene expression of a reporter gene (GUS pBI121) in embryogenic callus of alkaligrass. Varying pressure of helium and the distance of the calli in the chamber are also being avaluated for efficiency in transformation." |
Language: | English |
References: | 0 |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Brown, A., and H. G. Hughes. 1995. Transient Expression of Β-Glucuronidase (GUS) activity in embryonic callus tissue of alkaligrass (Puccinellia distans) using particle bombardment. HortScience. 30(4-7):p. 913 (1224). |
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| DOI: 10.21273/HORTSCI.30.4.913G |
| Web URL(s): https://journals.ashs.org/hortsci/view/journals/hortsci/30/4/article-p913G.xml Last checked: 11/14/2019 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: SB 1 .H64 |
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